Isolation and characterization of seven tetranucleotide microsatellite loci in mungbean, Vigna radiata

A simple and rapid method for isolating tetranucleotide microsatellites in mungbean, Vigna radiata, based on the 5'-anchored polymerase chain reaction technique, revealed 15 microsatellite sequences. We report on the characterization of seven polymorphic microsatellite loci in V. radiata. The number of alleles per locus ranged from 2 to 6, whereas the observed heterozygosity ranged from 0 to 0.561. Tetranucleotide markers are useful because they amplify fewer stutter bands thus making scoring easier. These markers will be useful for detecting genetic variation in mungbean varieties for germplasm management and development of the crop.