Protective effect of polysaccharides on simulated microgravity-induced functional inhibition of human NK cells

被引：64

作者：

Ting Huyan ^{[1
,2
]}

Li, Qi ^{[1
]}

Yang, Hui ^{[1
]}

Jin, Ming-Liang ^{[1
]}

Zhang, Ming-Jie ^{[1
,3
]}

Ye, Lin-Jie ^{[1
]}

Li, Ji ^{[1
]}

Huang, Qing-Sheng ^{[1
]}

Yin, Da-Chuan ^{[1
,2
]}

机构：

[1] Northwestern Polytech Univ, Sch Life Sci, Key Lab Space Biosci & Space Biotechnol, Xian 710072, Shaanxi, Peoples R China

[2] Northwestern Polytech Univ, Sch Mat Sci & Engn, Xian 710072, Shaanxi, Peoples R China

[3] US FDA, Mol Virol Lab, Ctr Biol Evaluat & Res, Rockville, MD 20857 USA

来源：

CARBOHYDRATE POLYMERS | 2014年 / 101卷

基金：

中国国家自然科学基金;

关键词：

Polysaccharide; Human natural killer (NK) cell; Cytotoxicity; Simulated microgravity (SMG); NATURAL-KILLER-CELLS; CYTOKINE PRODUCTION; D-FRACTION; RECEPTOR; CYTOTOXICITY; MUSHROOMS; ACTIVATE; TLR4; LIPOPOLYSACCHARIDE; MACROPHAGES;

D O I：

10.1016/j.carbpol.2013.10.021

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Polysaccharides are believed to be strong immunostimulants that can promote the proliferation and activity of T cells, B cells, macrophages and natural killer (NK) cells. This study aimed to investigate the effects of five polysaccharides (Grifola frondosa polysaccharide (GFP), lentinan (LNT), G. lucidum polysaccharide (GLP), Lycium barbarum polysaccharide (LBP) and yeast glucan (YG)) on primary human NK cells under normal or simulated microgravity (SMG) conditions. Our results demonstrated that polysaccharides markedly promoted the cytotoxicity of NK cells by enhancing IFN-gamma and perforin secretion and increasing the expression of the activating receptor NKp30 under normal conditions. Meanwhile polysaccharides can enhance NK cell function under SMG conditions by restoring the expression of the activating receptor NKG2D and reducing the early apoptosis and late apoptosis/necrosis. Moreover, the antibody neutralization test showed that CR3 may be the critical receptor involved in polysaccharides induced NK cells activation. These findings indicated that polysaccharides may be used as immune regulators to promote the health of the public and astronauts during space missions. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：819 / 827

页数：9

共 45 条

[21] Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method
Livak, KJ
Schmittgen, TD
[J]. METHODS, 2001, 25 (04) : 402 - 408
[22] Impact of simulated microgravity on cell cycle control and cytokine release by U937 cells
Maier, J. A. M.
[J]. INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY, 2006, 19 (02): : 279 - 286
[23] Effect of Microgravity on Immune Cell Viability and Proliferation Simulation Using 3-D Clinostat
Martinelli, Leonardo K.
Russomano, Thais
Dos Santos, Marlise A.
Falcao, Felipe P.
Bauer, Moises E.
Machado, Amanda
Sundaresan, Alamelu
[J]. IEEE ENGINEERING IN MEDICINE AND BIOLOGY MAGAZINE, 2009, 28 (04): : 85 - 90
[24] MATSUMOTO Y, 1991, J PROTOZOOL, V38, pS6
[25] MEEHAN RT, 1992, IMMUNOLOGY, V76, P491
[26] FimH Can Directly Activate Human and Murine Natural Killer Cells via TLR4
Mian, M. Firoz
Lauzon, Nicole M.
Andrews, David W.
Lichty, Brian D.
Ashkar, Ali A.
[J]. MOLECULAR THERAPY, 2010, 18 (07) : 1379 - 1388
[27] BIOCHEMICAL-CHARACTERIZATION OF A COMPONENT IN EXTRACTS OF VISCUM-ALBUM ENHANCING HUMAN NK CYTO-TOXICITY
MUELLER, EA
HAMPRECHT, K
ANDERER, FA
[J]. IMMUNOPHARMACOLOGY, 1989, 17 (01): : 11 - 18
[28] NANBA H, 1987, CHEM PHARM BULL, V35, P2459
[29] Physical contact between lipopolysaccharide and Toll-like receptor 4 revealed by genetic complementation
Poltorak, A
Ricciardi-Castagnoli, P
Citterio, S
Beutler, B
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (05) : 2163 - 2167
[30] Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice:: Mutations in Tlr4 gene
Poltorak, A
He, XL
Smirnova, I
Liu, MY
Van Huffel, C
Du, X
Birdwell, D
Alejos, E
Silva, M
Galanos, C
Freudenberg, M
Ricciardi-Castagnoli, P
Layton, B
Beutler, B
[J]. SCIENCE, 1998, 282 (5396) : 2085 - 2088

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