BrdU-, neuroD (nrd)- and Hu-studies reveal unusual non-ventricular neurogenesis in the postembryonic zebrafish forebrain

被引：93

作者：

Mueller, T ^{[1
]}

Wullimann, MF ^{[1
]}

机构：

[1] Univ Bremen, Brain Res Inst, D-28344 Bremen, Germany

来源：

MECHANISMS OF DEVELOPMENT | 2002年 / 117卷 / 1-2期

关键词：

zebrafish; Hu-protein; 5-bromo-2 '-deoxyuridine; proliferating cell nuclear antigen; neuroD; nrd; forebrain; diencephalon; preglomerular area; pretectum; posterior tuberculum; telencephalon; cerebellum; external granular layer; basic helix-loop-helix transcription factor; neurogenesis; mitosis; proliferation;

D O I：

10.1016/S0925-4773(02)00194-6

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In the postembryonic zebrafish forebrain, subpial locations of neurogenesis do exist in the early cerebellar external granular layer, and unusually among vertebrates - in the primordial pretectal (M1) and preglomerular (M2) Anlagen as shown here with 5-bromo-2'-deoxyuridine (BrdU)/Hu-immunocytochemistry and in situ hybridization of neuroD. An intermediate BrdU incubation time of 12-16 h reveals in addition to proliferative ventricularly located cells those in M1 and M2. This BrdU saturation-labeling shows - in conjunction with a Hu-assay demonstrating earliest neuronal differentiation - that proliferating cells in M1 and M2 represent neuronal progenitors. This is demonstrated by single BrdU-Iabeled and double BrdU-/Hu-labeled cells in these aggregates. Further, expression of NeuroD - a marker for freshly determined neuronal cells - confirms this unusual subpial postembryonic forebrain neurogenesis. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：123 / 135

页数：13

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