Chiral recognition in the binding of helicenediamine to double strand DNA: interactions between low molecular weight helical compounds and a helical polymer

被引：83

作者：

Honzawa, S

Okubo, H

Anzai, S

Yamaguchi, M ^{[1
]}

Tsumoto, K

Kumagai, I

机构：

[1] Tohoku Univ, Grad Sch Pharmaceut Sci, Dept Chim Organ, Sendai, Miyagi 9808578, Japan

[2] Tohoku Univ, Grad Sch Engn, Dept Biomol Engn, Sendai, Miyagi 9808579, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2002年 / 10卷 / 10期

基金：

日本学术振兴会;

关键词：

D O I：

10.1016/S0968-0896(02)00175-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Binding of a helicene, 5,8-bis(aminomethyl)-1,12-dimethylbenzo[c]phenanthrene, to calf thymus DNA was studied using UV, CD, and fluorescence spectroscopy as well as calorimetry. The enantiomeric helicenes strongly bound to the double strand DNA possessing the right-handed helical structure. In addition, chiral recognition was observed in the binding, where the (P)helicene with the right-handed helicity formed more stable complex than the (M)-helicene with the left-handed helicity. The binding studies of the helicenes and natural nucleosides by H-1 NMR spectroscopy also revealed the higher affinity to the (P)-helicene. Both monomeric and polymeric nucleic acids thus turned out to favor the (P)-helicity. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：3213 / 3218

页数：6

共 24 条

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