Isolation of a highly clonogenic and multipotential subfraction of adult stem cells from bone marrow stroma.

被引：171

作者：

Smith, JR ^{[1
]}

Pochampally, R ^{[1
]}

Perry, A ^{[1
]}

Hsu, SC ^{[1
]}

Prockop, DJ ^{[1
]}

机构：

[1] Tulane Univ, Hlth Sci Ctr, Ctr Gene Therapy, New Orleans, LA 70112 USA

来源：

STEM CELLS | 2004年 / 22卷 / 05期

关键词：

MSCs; mineralizing cells; adipocytes; RS cells;

D O I：

10.1634/stemcells.22-5-823

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Attempts have been made to develop cell and gene therapies using the adult stem cells from bone marrow referred to as mesenchymal stem cells or marrow stromal cells (MSCs). However, the results have been variable in part because there are no standardized protocols for preparing and characterizing MSCs. In the experiments presented here, we developed a standardized assay by light scattering to measure the content of rapidly self-renewing cells (RS cells) in preparations of MSCs. The assay quickly identifies preparations of MSCs that replicate rapidly in subsequent culture. In addition, the standardized assay enabled us to isolate RS cells that were up to 90% clonogenic and that generated single cell-derived colonies that differentiated into either mineralizing cells or adipocytes with appropriate additions to the medium.

引用

页码：823 / 831

页数：9

共 21 条

[1] MESENCHYMAL STEM-CELLS [J].

CAPLAN, AI .

JOURNAL OF ORTHOPAEDIC RESEARCH, 1991, 9 (05) :641-650

[2]

CASTROMALASPINA H, 1980, BLOOD, V56, P289

[3] Markers of senescence? [J].

Coates, PJ .

JOURNAL OF PATHOLOGY, 2002, 196 (04) :371-373

[4] Identification of a subpopulation of rapidly self-renewing and multipotential adult stem cells in colonies of human marrow stromal cells [J].

Colter, DC ;

Sekiya, I ;

Prockop, DJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (14) :7841-7845

[5]

COLTER DC, 2000, P NATL ACAD SCI USA, V96, P7292

[6] Propagation and senescence of human marrow stromal cells in culture: a simple colony-forming assay identifies samples with the greatest potential to propagate and differentiate [J].

DiGirolamo, CM ;

Stokes, D ;

Colter, D ;

Phinney, DG ;

Class, R ;

Prockop, DJ .

BRITISH JOURNAL OF HAEMATOLOGY, 1999, 107 (02) :275-281

[7] BONE-MARROW OSTEOGENIC STEM-CELLS - INVITRO CULTIVATION AND TRANSPLANTATION IN DIFFUSION-CHAMBERS [J].

FRIEDENSTEIN, AJ ;

CHAILAKHYAN, RK ;

GERASIMOV, UV .

CELL AND TISSUE KINETICS, 1987, 20 (03) :263-272

[8] CONFORMATION-SENSITIVE GEL-ELECTROPHORESIS FOR RAPID DETECTION OF SINGLE-BASE DIFFERENCES IN DOUBLE-STRANDED PCR PRODUCTS AND DNA FRAGMENTS - EVIDENCE FOR SOLVENT-INDUCED BENDS IN DNA HETERODUPLEXES [J].

GANGULY, A ;

ROCK, MJ ;

PROCKOP, DJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (21) :10325-10329

[9] Stromal mesenchymal progenitor cells [J].

Ghilzon, R ;

McCulloch, CAG ;

Zohar, R .

LEUKEMIA & LYMPHOMA, 1999, 32 (3-4) :211-221

[10] Dye efflux studies suggest that hematopoietic stem cells expressing low or undetectable levels of CD34 antigen exist in multiple species [J].

Goodell, MA ;

Rosenzweig, M ;

Kim, H ;

Marks, DF ;

DeMaria, M ;

Paradis, G ;

Grupp, SA ;

Sieff, CA ;

Mulligan, RC ;

Johnson, RP .

NATURE MEDICINE, 1997, 3 (12) :1337-1345

← 1 2 3 →