The interface of σ with core RNA polymerase is extensive, conserved, and functionally specialized

被引:132
作者
Sharp, MM
Chan, CL
Lu, CZ
Marr, MT
Nechaev, S
Merritt, EW
Severinov, K
Roberts, JW
Gross, CA [1 ]
机构
[1] Univ Calif San Francisco, San Francisco, CA 94143 USA
[2] Cornell Univ, Biochem Mol & Cell Biol Sect, Ithaca, NY 14853 USA
[3] Rutgers State Univ, Waksman Inst, Dept Genet, Piscataway, NJ 08854 USA
[4] Proctor & Gamble Pharmaceut, Hlth Care Res Ctr, Mason, OH 45040 USA
关键词
sigma factor; core; RNA polymerase; transcription; initiation; pausing;
D O I
10.1101/gad.13.22.3015
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The sigma subunit of eubacterial RNA polymerase is required throughout initiation, but how it communicates with core polymerase (alpha(2)beta beta') is poorly understood. The present work addresses the location and function of the interface of sigma with core. Our studies suggest that this interface is extensive as mutations in six conserved regions of sigma(70) hinder the ability of sigma to bind core. Direct binding of one of these regions to core can be demonstrated using a peptide-based approach. The same regions, and even equivalent residues, in sigma(32) and sigma(70) alter core interaction, suggesting that sigma(70) family members use homologous residues, at least in part, to interact with core. Finally, the regions of sigma that we identify perform specialized functions, suggesting that different portions of the interface perform discrete roles during transcription initiation.
引用
收藏
页码:3015 / 3026
页数:12
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