Determination of isomers of inositol mono- to hexaphosphates in selected foods and intestinal contents using high-performance ion chromatography

被引：107

作者：

Skoglund, E

Carlsson, NG

Sandberg, AS

机构：

[1] Department of Food Science, Chalmers University of Technology

[2] Department of Food Science, Chalmers University of Technology, Box 5401

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1997年 / 45卷 / 02期

关键词：

inositol phosphate isomers; high-performance ion chromatography; HPIC; food; intestinal content;

D O I：

10.1021/jf9603238

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A sensitive high-performance ion chromatography (HPIC) method for separation and quantitative determination of inositol mono- to hexaphosphate (IP1-IP6) isomers in selected foods and intestinal contents is described. The method includes extraction of samples with HCl, separation of the inositol phosphates from the crude extract by anion-exchange chromatography, separation on highperformance ion-exchange columns with gradient elution in two combined HPIC systems, and detection using either postcolumn reaction and UV detection (system 1) or chemically suppressed conductivity detection (system 2). IP2-IP6 in samples were determined on the first system, which also separated isomers of IP4 and IP5. Fractions of IP1-IP3 from the first system were transferred to the second system after some additional sample preparation and analyzed for isomers, The applicability and sensitivity of the method are illustrated by measuring the content of inositol phosphates in foods, ileal contents, and feces of human balances.

引用

页码：431 / 436

页数：6

共 28 条

[21] HPLC METHOD FOR DETERMINATION OF INOSITOL TRIPHOSPHATES, TETRAPHOSPHATES PENTAPHOSPHATES AND HEXAPHOSPHATES IN FOODS AND INTESTINAL CONTENTS [J].