Rapid detection of tetramethylenedisulfotetramine in human blood by solid-phase microextraction/gas chromatography

The solid-phase microextraction (SPME) coupled with gas chromatography (GC) was used to develop a simple, rapid and sensitive method for the determination of tetramethylenedisulfotetramine (tetramine) in human blood. The tetramine was extracted with a fused silica fibre coated with a 100 mu m polydimethylsiloxane (PDMS) and detected by GC with a nitrogen/phosphorus detector (NPD). The effects of extraction and desorption times as well as the extraction temperature were studied. A linear response of tetramine over a concentration range of 0.008-0.5 mu g ml(-1) with a correlation coefficient of 0.998 and detection limit 0.001 mu g ml(-1) (3s criteria) were obtained. Spiked human blood samples were analyzed. The analytical recoveries ranged from 92 to 114% and the mean RSD was 10.5%. (C)2000 Elsevier Science B.V. All rights reserved.