SOLID-PHASE MICROEXTRACTION FOR THE DETERMINATION OF THE FREE CONCENTRATION OF VALPROIC ACID IN HUMAN PLASMA BY CAPILLARY GAS-CHROMATOGRAPHY

The potential of solid-phase microextraction in the bioanalysis of drugs is demonstrated. The free concentration of valproic acid in human plasma was determined by equilibrium dialysis followed by solid-phase microextraction and capillary gas chromatography. Human plasma samples were dialysed at room temperature. To the dialysate was added an internal standard and the pH was adjusted to 2.5. The polymethylsiloxane-coated fused-silica fibre of the solid-phase microextraction device was inserted into the dialysate for 3 min. The sorbed analytes were then thermally desorbed at 210 degrees C in the split-splitless injection port of the gas chromatograph, separated on a Nukol capillary column and detected with a flame ionization detector. The method was shown to be highly reproducible with a detection limit of 1 mu g/ml of free valproic acid in human plasma.