Determination of glucose using bienzyme layered assembly magnetoelastic sensing device

被引：42

作者：

Pang, Pengfei ^{[1
,2
]}

Zhang, Yanli ^{[2
]}

Ge, Shutian ^{[1
]}

Cai, Qingyun ^{[1
]}

Yao, Shouzhuo ^{[1
]}

Grimes, Craig A. ^{[3
,4
]}

机构：

[1] Hunan Univ, State Key Lab Chemo, Dept Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China

[2] Yunnan Nationalities Univ, Sch Chem & Biotechnol, Kunming 650031, Peoples R China

[3] Penn State Univ, Dept Elect Engn, University Pk, PA 16802 USA

[4] Penn State Univ, Dept Mat Sci & Engn, University Pk, PA 16802 USA

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2009年 / 136卷 / 02期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

Magnetoelastic sensor; Glucose; Horseradish peroxidase (HRP); Glucose oxidase (GOx); 3,3 ',5,5 '-Tetramethylbenzidine (TMB); LANGMUIR-BLODGETT-FILMS; MOLECULAR FILMS; SENSORS; BIOSENSOR; OXIDASE; WIRELESS; ADSORPTION; POLYMER; SYSTEM;

D O I：

10.1016/j.snb.2008.11.044

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A bienzyme layered assembly on magnetoelastic sensor, consisting of horseradish peroxidase and glucose oxidase is used to sense glucose by the horseradish peroxidase-mediated oxidation of 3,3',5,5'-tetramethylbenzidine. by H2O2, and the formation of the insoluble product on the sensor surface. A horseradish peroxidase is used to analyze hydrogen peroxide (H2O2) via the biocatalyzed oxidation of tetra methylbenzidine and the precipitation of the insoluble product. The glucose oxidase catalyzed oxidation of glucose produces gluconic acid and H2O2, and the generated H2O2 effects the formation of the insoluble product in the presence of horseradish peroxidase. The insoluble product accumulated on the sensor surface resulting in shifts of the sensor resonance frequency which are correlated with the amount of H2O2 or glucose. The biosensor response is linear in the range of glucose concentrations of 5-50 mM, with a detection limit of 2 mM at a noise level of similar to 10 Hz. The biosensor is applied to determine glucose concentration in urine sample. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：310 / 314

页数：5

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