Molecular cloning and functional expression of Cav3.1c, a T-type calcium channel from human brain

被引：54

作者：

Cribbs, LL

Gomora, JC

Daud, AN

Lee, JH

Perez-Reyes, E

机构：

[1] Loyola Univ, Med Ctr, Dept Physiol, Maywood, IL 60153 USA

[2] Loyola Univ, Med Ctr, Cardiovasc Inst, Maywood, IL 60153 USA

来源：

FEBS LETTERS | 2000年 / 466卷 / 01期

关键词：

calcium channel; molecular cloning;

D O I：

10.1016/S0014-5793(99)01756-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Low voltage-activated T-type calcium channels are encoded by a family of at least three genes, with additional diversity created by alternative splicing, This study describes the cloning of the human brain alpha 1G, which is a novel isoform, Ca(v)3.1c, Comparison of this sequence to genomic sequences deposited in the GenBank allowed us to identify the intron/exon boundaries of the human CACNA1G gene, A full-length cDNA mas constructed, then used to generate a stably-transfected mammalian cell line. The resulting currents were analyzed for their voltage- and time-dependent properties, These properties identify this gene as encoding a T-type Ca2+ channel. (C) 2000 Federation of European Biochemical Societies.

引用

页码：54 / 58

页数：5

共 17 条

[11] Voltage-gated ion channels and hereditary disease [J].