Selection of methylation procedures for quantitation of short-chain glycerol-bound compounds formed during thermoxidation

被引：36

作者：

Berdeaux, O ^{[1
]}

Marquez-Ruiz, G ^{[1
]}

Dobarganes, C ^{[1
]}

机构：

[1] CSIC, Inst Grasa, Seville 41012, Spain

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 863卷 / 02期

关键词：

methylation; fatty acids; aldehydic acids; acetals;

D O I：

10.1016/S0021-9673(99)00974-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Five methylation procedures, including base- and acid-catalyzed methods, were tested in thermoxidized methyl linoleate and trilinolein, in order to quantitate major oxidation short-chain glycerol-bound compounds by gas chromatography. Results indicated that transmethylations using KOH in methanol or CH3ONa-CH3OH in tert.-butylmethyl ether were the most appropriate methods, given the excellent reproducibility and practically complete recovery obtained for the compounds of interest, mainly short-chain fatty acids and aldehydic acids. Also, formation of acids from aldehydes during thermoxidation as well as modifications of aldehydic functions under acidic conditions, such as conversion to acetals, were checked using dodecanal as model aldehyde. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：171 / 181

页数：11

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