Genetic differentiation of Phoma exigua varieties by means of AFLP fingerprints

被引:32
作者
Abeln, ECA
Stax, AM
de Gruyter, J
van der AA, HA
机构
[1] Cent Bur Schimmelcultures, NL-3508 AD Utrecht, Netherlands
[2] Plant Protect Serv, NL-6700 HC Wageningen, Netherlands
来源
MYCOLOGICAL RESEARCH | 2002年 / 106卷
关键词
D O I
10.1017/S0953756202005804
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Several varieties have been distinguished within the phytopathogenic pycnidial fungus Phoma exigua on the basis of cultural characteristics. These varieties have been stated to correlate with differences in ability to attack different host plants. Molecular discrimination of the varieties was investigated using amplified fragment length polymorphism (AFLP) and sequence analysis of the ITS region of the rDNA cluster on a set of 43 strains and 7 outgroup strains. The ITS sequences of the 43 different P. exigua strains were highly similar and revealed no subgroups within P. exigua while the AFLP fingerprint patterns of two primer combinations showed clear clustering of most varieties.
引用
收藏
页码:419 / 427
页数:9
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