Simultaneous analysis of nine active components in Gegen Qinlian preparations by high-performance liquid chromatography with diode array detection

被引：23

作者：

Chen, Lihong

Wang, Qiang

Liu, Jining

机构：

[1] China Pharmaceut Univ, Dept Chinese Meteria Med Anal, Key Lab Modern Tradit Chinese Med, Nanjing 210038, Peoples R China

[2] Nanjing Inst Environm Sci, State Environm Protect Adm China, Nanjing, Peoples R China

来源：

JOURNAL OF SEPARATION SCIENCE | 2006年 / 29卷 / 14期

关键词：

alkaloids; flavonoids; Gegen Qinlian preparations; saponins; simultaneous analysis;

D O I：

10.1002/jssc.200600123

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学]; 081704 [应用化学];

摘要：

HPLC with diode array detection (HPLC/DAD) was employed to determine the quantities of puerarin, daidzin, daidzein, berberine, palmatine, coptisine, baicalin, baicalein, and glycyrrhizin in Gegen Qinlian preparations of three different pharmaceutical forms including decoction, dispensing granule and pill. The calibration curves for the nine bioactive components were linear in the given concentration ranges. The precision of the method was in the range of 0.2 - 5.0% (RSD), and the recoveries of this method were between 96.5 and 104.1%. The proposed method was applicable to analyze Gegen Quilian preparations.

引用

页码：2203 / 2210

页数：8

共 23 条

[1]

Determination of saikosaponin derivatives in Radix bupleuri and in pharmaceuticals of the Chinese multiherb remedy Xiaochaihu-tang using liquid chromatographic tandem mass spectrometry [J].