Cyclic nucleotide content of tobacco BY-2 cells

被引：25

作者：

Richards, H

Das, S

Smith, CJ

Pereira, L

Geisbrecht, A

Devitt, NJ

Games, DE

van Geyschem, J

Brenton, AG

Newton, RP

机构：

[1] Univ Wales Swansea, Biochem Grp, Swansea SA2 8PP, W Glam, Wales

[2] Univ Wales Swansea, Dept Chem, Mass Spectrometry Res Unit, Swansea SA2 8PP, W Glam, Wales

来源：

PHYTOCHEMISTRY | 2002年 / 61卷 / 05期

关键词：

tobacco; BY2; cyclic AMP; cyclic GMP; cyclic CMP; mass spectrometry; electrospray;

D O I：

10.1016/S0031-9422(02)00266-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cyclic nucleotide content of cultured tobacco bright yellow-2 (BY-2) cells was determined, after freeze-killing, perchlorate extraction and sequential chromatography, by radioimmunoassay. The identities of the putative cyclic nucleotides, adenosine 3',5'-cyclic monophosphate (cyclic AMP), guanosine 3',5'-cyclic monophosphate (cyclic GMP) and cytidine 3,5'-cyclic monophosphate (cyclic CMP) were unambiguously confirmed by tandem mass spectrometry. The potential of BY-2 cell cultures as a model system for future investigations of cyclic nucleotide function in higher plants is discussed. (C) 2002 Elsevier Science Ltd. All rights reserved.

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页码：531 / 537

页数：7

相关论文

共 54 条

[1] EVIDENCE AGAINST OCCURRENCE OF ADENOSINE-3'-5'-CYCLIC MONOPHOSPHATE IN HIGHER-PLANTS [J].

AMRHEIN, N .

PLANTA, 1974, 118 (03) :241-258

[2] FUNCTIONAL EXPRESSION OF A PROBABLE ARABIDOPSIS-THALIANA POTASSIUM CHANNEL IN SACCHAROMYCES-CEREVISIAE [J].

ANDERSON, JA ;

HUPRIKAR, SS ;

KOCHIAN, LV ;

LUCAS, WJ ;

GABER, RF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (09) :3736-3740

[3] Differential interaction of branch-specific inhibitors of isoprenoid biosynthesis with cell cycle progression in tobacco BY-2 cells [J].

Andréa, H ;

Fischt, I ;

Bach, TJ .

PHYSIOLOGIA PLANTARUM, 2000, 110 (03) :342-349

[4] Early signalling events in the apoplastic oxidative burst in suspension cultured French bean cells involve cAMP and Ca2+ [J].

Bindschedler, LV ;

Minibayeva, F ;

Gardner, SL ;

Gerrish, C ;

Davies, DR ;

Bolwell, GP .

NEW PHYTOLOGIST, 2001, 151 (01) :185-194

[5] CYCLIC-AMP, THE RELUCTANT MESSENGER IN PLANTS [J].

BOLWELL, GP .

TRENDS IN BIOCHEMICAL SCIENCES, 1995, 20 (12) :492-495

[6] Role of active oxygen species and NO in plant defence responses [J].

Bolwell, GP .

CURRENT OPINION IN PLANT BIOLOGY, 1999, 2 (04) :287-294

[7] PHYTOCHROME SIGNAL-TRANSDUCTION PATHWAYS ARE REGULATED BY RECIPROCAL CONTROL MECHANISMS [J].

BOWLER, C ;

YAMAGATA, H ;

NEUHAUS, G ;

CHUA, NH .

GENES & DEVELOPMENT, 1994, 8 (18) :2188-2202

[8] CYCLIC-GMP AND CALCIUM MEDIATE PHYTOCHROME PHOTOTRANSDUCTION [J].

BOWLER, C ;

NEUHAUS, G ;

YAMAGATA, H ;

CHUA, NH .

CELL, 1994, 77 (01) :73-81

[9] INFLUENCE OF LIGHT ON CYCLIC-NUCLEOTIDE METABOLISM IN PLANTS - EFFECT OF DIBUTYRYL CYCLIC-NUCLEOTIDES ON CHLOROPLAST COMPONENTS [J].

BROWN, EG ;

NEWTON, RP ;

EVANS, DE ;

WALTON, TJ ;

YOUNIS, LM ;

VAUGHAN, JM .

PHYTOCHEMISTRY, 1989, 28 (10) :2559-2563

[10] PATHOGENIC ELICITATION OF PHYTOALEXIN IN LUCERNE TISSUES - INVOLVEMENT OF CYCLIC-AMP IN INTRACELLULAR MECHANISM [J].

COOKE, CJ ;

NEWTON, RP ;

SMITH, CJ ;

WALTON, TJ .

BIOCHEMICAL SOCIETY TRANSACTIONS, 1989, 17 (05) :915-916

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