Cyclic nucleotide content of tobacco BY-2 cells

被引：25

作者：

Richards, H

Das, S

Smith, CJ

Pereira, L

Geisbrecht, A

Devitt, NJ

Games, DE

van Geyschem, J

Brenton, AG

Newton, RP

机构：

[1] Univ Wales Swansea, Biochem Grp, Swansea SA2 8PP, W Glam, Wales

[2] Univ Wales Swansea, Dept Chem, Mass Spectrometry Res Unit, Swansea SA2 8PP, W Glam, Wales

来源：

PHYTOCHEMISTRY | 2002年 / 61卷 / 05期

关键词：

tobacco; BY2; cyclic AMP; cyclic GMP; cyclic CMP; mass spectrometry; electrospray;

D O I：

10.1016/S0031-9422(02)00266-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cyclic nucleotide content of cultured tobacco bright yellow-2 (BY-2) cells was determined, after freeze-killing, perchlorate extraction and sequential chromatography, by radioimmunoassay. The identities of the putative cyclic nucleotides, adenosine 3',5'-cyclic monophosphate (cyclic AMP), guanosine 3',5'-cyclic monophosphate (cyclic GMP) and cytidine 3,5'-cyclic monophosphate (cyclic CMP) were unambiguously confirmed by tandem mass spectrometry. The potential of BY-2 cell cultures as a model system for future investigations of cyclic nucleotide function in higher plants is discussed. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

收藏

页码：531 / 537

页数：7

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