CUL-2 is required for the G1-to-S-phase transition and mitotic chromosome condensation in Caenorhabditis elegans

被引：107

作者：

Feng, H ^{[1
]}

Zhong, WW ^{[1
]}

Punkosdy, G ^{[1
]}

Gu, SB ^{[1
]}

Zhou, L ^{[1
]}

Seabolt, EK ^{[1
]}

Kipreos, ET ^{[1
]}

机构：

[1] Univ Georgia, Dept Cell Biol, Athens, GA 30602 USA

来源：

NATURE CELL BIOLOGY | 1999年 / 1卷 / 08期

关键词：

D O I：

10.1038/70272

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The human cullin protein CUL-2 functions in a ubiquitin-ligase complex with the von Hippel-Lindau (VHL) tumour suppressor protein. Here we show that, in Caenorhabditis elegans, cul-2 is expressed in proliferating cells and is required at two distinct points in the cell cycle, the G1-to-S-phase transition and mitosis. cul-2 mutant germ cells undergo a G1-phase arrest that correlates with accumulation of CKI-1, a member of the CIP/KIP family of cyclin-dependent-kinase inhibitors. In cul-2 mutant embryos, mitotic chromosomes are unable to condense, leading to unequal DNA segregation, chromosome bridging and the formation of multiple nuclei.

引用

页码：486 / 492

页数：7

共 29 条

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