Protein adsorption and separation with chitosan-based amphoteric membranes

被引：75

作者：

Feng, Zhicheng ^{[1
]}

Shao, Zhengzhong ^{[1
]}

Yao, Jinrong ^{[1
]}

Huang, Yufang ^{[2
]}

Chen, Xin ^{[1
]}

机构：

[1] Fudan Univ, Adv Mat Lab, Dept Macromol Sci, Key Lab Mol Engn Polymers MOE, Shanghai 200433, Peoples R China

[2] Fudan Univ, Natl Microanal Ctr, Dept Mat Sci, Shanghai 200433, Peoples R China

来源：

POLYMER | 2009年 / 50卷 / 05期

基金：

中国国家自然科学基金;

关键词：

Natural polymer; Amphoteric material; Protein separation; ION-EXCHANGE MEMBRANES; AFFINITY MEMBRANES; LYSOZYME ADSORPTION; HOLLOW-FIBER; ACID; CHROMATOGRAPHY; PURIFICATION; EQUILIBRIUM; REMOVAL; MODULES;

D O I：

10.1016/j.polymer.2008.12.046

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

A macropurous amphoteric membrane was successfully prepared by solution blending of a natural polymer chitosan (CS) and its derivative carboxymethylchitosan (CMCS). The adsorption of two model proteins (ovalbumin and lysozyme) with very different pH, values on this CS/CMCS blend membrane was investigated in batch systems. The results showed that both proteins could be effectively adsorbed on the membrane, but the adsorption capacities were influenced by the pH, the initial protein concentration and the CMCS content in the membrane. Because of the amphoteric nature of the protein and the CS/CMCS membrane, the pH for the maximum adsorption of ovalbumin and lysozyme was different, which is the basis for the separation of these proteins from binary mixtures. As the CS/CMCS blend membrane also showed good desorption properties for those two proteins, both ovalbumin and lysozyme were successfully separated from binary mixtures by adjusting only the pH of the feed and the desorption solutions. (c) 2009 Elsevier Ltd. All rights reserved.

引用

页码：1257 / 1263

页数：7

共 45 条

[1] Biosorption of reactive dyes by dried activated sludge: equilibrium and kinetic modelling [J].

Aksu, Z .

BIOCHEMICAL ENGINEERING JOURNAL, 2001, 7 (01) :79-84

[2] Evaluation of the adsorption kinetics and equilibrium for the potential removal of acid dyes using a biosorbent [J].

Arami, Mokhtar ;

Limaee, Nargess Yousefi ;

Mahmoodi, Niyaz Mohammad .

CHEMICAL ENGINEERING JOURNAL, 2008, 139 (01) :2-10

[3] Studies of adsorption of alkaline trypsin by poly(methacrylic acid) brushes on chitosan membranes [J].

Bayramoglu, Guelay ;

Erdogan, Hakan ;

Arica, M. Yakup .

JOURNAL OF APPLIED POLYMER SCIENCE, 2008, 108 (01) :456-465

[4] Influence of surface charge on protein adsorption at an amphoteric surface: Effects of varying acid to base ratio [J].

Burns, NL ;

Holmberg, K ;

Brink, C .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1996, 178 (01) :116-122

[5] Modification of porous alumina membranes with n-alkanoic acids and their application in protein adsorption [J].

Chang, CS ;

Suen, SY .

JOURNAL OF MEMBRANE SCIENCE, 2006, 275 (1-2) :70-81

[6] PROTEIN-A IMMUNOAFFINITY HOLLOW-FIBER MEMBRANES FOR IMMUNOGLOBULIN-G PURIFICATION - EXPERIMENTAL CHARACTERIZATION [J].

CHARCOSSET, C ;

SU, ZG ;

KAROOR, S ;

DAUN, G ;

COLTON, CK .

BIOTECHNOLOGY AND BIOENGINEERING, 1995, 48 (04) :415-427

[7] Macroporous chitosan/carboxymethylclellulose blend membranes and their application for lysozyme adsorption [J].

Chen, X ;

Liu, JH ;

Feng, ZC ;

Shao, ZZ .

JOURNAL OF APPLIED POLYMER SCIENCE, 2005, 96 (04) :1267-1274

[8]

Chen X, 1999, J APPL POLYM SCI, V73, P975, DOI 10.1002/(SICI)1097-4628(19990808)73:6<975::AID-APP15>3.0.CO

[9]

2-#

[10]

Chen X, 2000, ACTA CHIM SINICA, V58, P1654

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