Zika Virus RNA Replication and Persistence in Brain and Placental Tissue

被引：167

作者：

Bhatnagar, Julu ^{[1
,5
]}

Rabeneck, Demi B. ^{[1
]}

Martines, Roosecelis B. ^{[1
]}

Reagan-Steiner, Sarah ^{[1
]}

Ermias, Yokabed ^{[1
]}

Estetter, Lindsey B. C. ^{[1
]}

Suzuki, Tadaki ^{[1
]}

Ritter, Jana ^{[1
]}

Keating, M. Kelly ^{[1
]}

Hale, Gillian ^{[1
]}

Gary, Joy ^{[1
]}

Muehlenbachs, Atis ^{[1
]}

Lambert, Amy ^{[2
]}

Lanciotti, Robert ^{[2
]}

Oduyebo, Titilope ^{[1
]}

Meaney-Delman, Dana ^{[1
]}

Bolanos, Fernando ^{[3
]}

Saad, Edgar Alberto Parra ^{[4
]}

Shieh, Wun-Ju ^{[1
]}

Zaki, Sherif R. ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, 1600 Clifton Rd NE,Mailstop G32, Atlanta, GA 30329 USA

[2] Ctr Dis Control & Prevent, Ft Collins, CO USA

[3] Patol Hosp Univ Neiva, Neiva, Colombia

[4] Inst Nacl Salud, Bogota, Colombia

[5] CDC, Mol Pathol, Infect Dis Pathol Branch, Div High Consequence Pathogens & Pathol,Natl Ctr, Atlanta, GA 30333 USA

来源：

EMERGING INFECTIOUS DISEASES | 2017年 / 23卷 / 03期

关键词：

RT-PCR; INFECTION; PREGNANCY; ANTIBODY; DEFECTS; BRAZIL;

D O I：

10.3201/eid2303.161499

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Zika virus is causally linked with congenital microcephaly and may be associated with pregnancy loss. However, the mechanisms of Zika virus intrauterine transmission, replication, and tropism and persistence in tissues are poorly understood. We tested tissues from 52 case-patients: 8 infants with microcephaly who died and 44 women suspected of being infected with Zika virus during pregnancy. By reverse transcription PCR, tissues from 32 (62%) case-patients (brains from 8 infants with microcephaly and placental/fetal tissues from 24 women) were positive for Zika virus. In situ hybridization localized replicative Zika virus RNA in brains of 7 infants and in placentas of 9 women who had pregnancy losses during the first or second trimester. These findings demonstrate that Zika virus replicates and persists in fetal brains and placentas, providing direct evidence of its association with microcephaly. Tissue-based reverse transcription PCR extends the time frame of Zika virus detection in congenital and pregnancy-associated infections.

引用

页码：405 / 414

页数：10

共 40 条

[1] Placental Mechanics in the Zika-Microcephaly Relationship [J].

Adibi, Jennifer J. ;

Zhao, Yaqi ;

Cartus, Abigail R. ;

Gupta, Phalguni ;

Davidson, Lance A. .

CELL HOST & MICROBE, 2016, 20 (01) :9-11

[2]

Aliota MT., 2016, PLoS Negl Trop Dis, V10

[3] Persistence of West Nile Virus in the Central Nervous System and Periphery of Mice [J].

Appler, Kim K. ;

Brown, Ashley N. ;

Stewart, Barbara S. ;

Behr, Melissa J. ;

Demarest, Valerie L. ;

Wong, Susan J. ;

Bernard, Kristen A. .

PLOS ONE, 2010, 5 (05)

[4] Type III Interferons Produced by Human Placental Trophoblasts Confer Protection against Zika Virus Infection [J].

Bayer, Avraham ;

Lennemann, Nicholas J. ;

Ouyang, Yingshi ;

Bramley, John C. ;

Morosky, Stefanie ;

De Azeved Marques, Ernesto Torres, Jr. ;

Cherry, Sara ;

Sadovsky, Yoel ;

Coyne, Carolyn B. .

CELL HOST & MICROBE, 2016, 19 (05) :705-712

[5] Detection of West Nile virus in formalin-fixed, paraffin-embedded human tissues by RT-PCR: A useful adjunct to conventional tissue-based diagnostic methods [J].

Bhatnagar, Julu ;

Guarner, Jeannette ;

Paddock, Christopher D. ;

Shieh, Wun-Ju ;

Lanciotti, Robert S. ;

Marfin, Anthony A. ;

Campbell, Grant L. ;

Zaki, Sherif R. .

JOURNAL OF CLINICAL VIROLOGY, 2007, 38 (02) :106-111

[6] Molecular Detection and Typing of Dengue Viruses from Archived Tissues of Fatal Cases by RT-PCR and Sequencing: Diagnostic and Epidemiologic Implications [J].

Bhatnagar, Julu ;

Blau, Dianna M. ;

Shieh, Wun-Ju ;

Paddock, Christopher D. ;

Drew, Clifton ;

Liu, Lindy ;

Jones, Tara ;

Patel, Mitesh ;

Zaki, Sherif R. .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2012, 86 (02) :335-340

[7] Zika Virus Infection in Pregnant Women in Rio de Janeiro [J].

Brasil, P. ;

Pereira, J. P., Jr. ;

Moreira, M. E. ;

Ribeiro Nogueira, R. M. ;

Damasceno, L. ;

Wakimoto, M. ;

Rabello, R. S. ;

Valderramos, S. G. ;

Halai, U. -A. ;

Salles, T. S. ;

Zin, A. A. ;

Horovitz, D. ;

Daltro, P. ;

Boechat, M. ;

Gabaglia, C. Raja ;

Carvalho de Sequeira, P. ;

Pilotto, J. H. ;

Medialdea-Carrera, R. ;

Cotrim da Cunha, D. ;

Abreu de Carvalho, L. M. ;

Pone, M. ;

Machado Siqueira, A. ;

Calvet, G. A. ;

Rodrigues Baiao, A. E. ;

Neves, E. S. ;

Nassar de Carvalho, P. R. ;

Hasue, R. H. ;

Marschik, P. B. ;

Einspieler, C. ;

Janzen, C. ;

Cherry, J. D. ;

Bispo de Filippis, A. M. ;

Nielsen-Saines, K. .

NEW ENGLAND JOURNAL OF MEDICINE, 2016, 375 (24) :2321-2334

[8] Development and evaluation of serotype- and group-specific fluorogenic reverse transcriptase PCR (TaqMan) assays for dengue virus [J].

Callahan, JD ;

Wu, SJL ;

Dion-Schultz, A ;

Mangold, BE ;

Peruski, LF ;

Watts, DM ;

Porter, KR ;

Murphy, GR ;

Suharyono, W ;

King, CC ;

Hayes, CG ;

Temenak, JJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (11) :4119-4124

[9] Detection and sequencing of Zika virus from amniotic fluid of fetuses with microcephaly in Brazil: a case study [J].

Calvet, Guilherme ;

Aguiar, Renato S. ;

Melo, Adriana S. O. ;

Sampaio, Simone A. ;

de Filippis, Ivano ;

Fabri, Allison ;

Araujo, Eliane S. M. ;

de Sequeira, Patricia C. ;

de Mendonca, Marcos C. L. ;

de Oliveira, Louisi ;

Tschoeke, Diogo A. ;

Schrago, Carlos G. ;

Thompson, Fabiano L. ;

Brasil, Patricia ;

dos Santos, Flavia B. ;

Nogueira, Rita M. R. ;

Tanurit, Amilcar ;

de Filippis, Ana M. B. .

LANCET INFECTIOUS DISEASES, 2016, 16 (06) :653-660

[10] Association between Zika virus and microcephaly in French Polynesia, 2013-15: a retrospective study [J].

Cauchemez, Simon ;

Besnard, Marianne ;

Bompard, Priscillia ;

Dub, Timothee ;

Guillemette-Artur, Prisca ;

Eyrolle-Guignot, Dominique ;

Salje, Henrik ;

Van Kerkhove, Maria D. ;

Abadie, VRonique ;

Garel, Catherine ;

Fontanet, Arnaud ;

Mallet, Henri-Pierre .

LANCET, 2016, 387 (10033) :2125-2132

← 1 2 3 4 →