RNA interference demonstrates a novel role for H2A.Z in chromosome segregation

被引：193

作者：

Rangasamy, D ^{[1
]}

Greaves, I ^{[1
]}

Tremethick, DJ ^{[1
]}

机构：

[1] Australian Natl Univ, John Curtin Sch Med Res, Canberra, ACT 2601, Australia

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2004年 / 11卷 / 07期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1038/nsmb786

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The histone variant H2A. Z plays an essential role in metazoans but its function remains to be determined. Here, we developed a new inducible RNAi strategy to elucidate the role of H2A. Z in mammalian cell lines. We show that in the absence of H2A. Z, the genome becomes highly unstable and that this instability is caused by defects in the chromosome segregation process. Analysis of H2A. Z localization reveals that in these cells it is enriched at heterochromatic foci with HP1alpha on the arms of chromosomes but not at centromeric regions. When H2A. Z is depleted, normal HP1alpha-chromatin interactions are disrupted on the chromosomal arms and, notably, also at pericentric regions. Therefore, H2A. Z controls the localization of HP1alpha. We conclude that H2A. Z is essential for the accurate transmission of chromosomes.

引用

收藏

页码：650 / 655

页数：6

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