Controlling the hydration of covalently immobilised penicillin G amidase in low-water medium: properties and use of Celite R-640

被引：15

作者：

Basso, A ^{[1
]}

De Martin, L ^{[1
]}

Ebert, C ^{[1
]}

Gardossi, L ^{[1
]}

Linda, P ^{[1
]}

机构：

[1] Univ Trieste, Dipartimento Sci Farmaceut, I-34127 Trieste, Italy

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2000年 / 8卷 / 4-6期

关键词：

celite; penicillin amidase; organic solvent; control of water activity;

D O I：

10.1016/S1381-1177(99)00075-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The study describes how Celite R-640 adsorbs liquid water in toluene and vapour water from a gas phase. In toluene, Celite R-640 is able to maintain the water activity (a(w)) constant within broad ranges of water concentrations. The a(w) values are strongly related to the original hydration of the Celite batches, but prolonged drying confers comparable and reproducible properties to the different batches. The use of Celite R-640 in controlling the hydration and activity of covalently immobilised PGA in toluene is reported. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：245 / 253

页数：9

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