Comparison of protein adsorption by anion-exchange interaction onto porous hollow-fiber membrane and gel bead-packed bed

Protein collection performance by anion-exchange interaction was compared between a single porous hollow-fiber membrane and a gel bead-packed bed under identical conditions from a viewpoint of flow resistance and breakthrough behavior. At a space velocity (SV) of 100 h(-1), an ethanolamino-group-containing porous hollow-fiber membrane (EA-C fiber) exhibited the same dynamic binding capacity for bovine serum albumin as a bed charged with diethylaminoethyl-group-containing gel beads (DEAE-G bed). Because mass transfer of the protein was enhanced by convective flow of the protein solution through the pores of the EA-C fiber, the EA-C fiber exhibited a higher productivity than the DEAE-G bed over SV = 100 h(-1).