Binding and uptake of surfactant protein D by freshly isolated rat alveolar type II cells

被引:17
作者
Herbein, JF
Savov, J
Wright, JR
机构
[1] Duke Univ, Med Ctr, Dept Cell Biol, Durham, NC 27710 USA
[2] Vet Affairs Med Ctr, Dept Med, Durham, NC 27705 USA
关键词
lung; lamellar bodies; surfactant lipids; alveolar macrophages; surfactant recycling;
D O I
10.1152/ajplung.2000.278.4.L830
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Alveolar type II cells secrete, internalize, and recycle pulmonary surfactant, a lipid and protein complex that increases alveolar compliance and participates in pulmonary host defense. Surfactant protein (SP) D, a collagenous C-type lectin, has recently been described as a modulator of surfactant homeostasis. Mice lacking SP-D accumulate surfactant in their alveoli and type II cell lamellar bodies, organelles adapted for recycling and secretion of surfactant. The goal of current study was to characterize the interaction of SP-D with rat type II cells. Type II cells bound SP-D in a concentration-, time-, temperature-, and calcium-dependent manner. However, SP-D binding did not alter type II cell surfactant lipid uptake. Type II cells internalized SP-D into lamellar bodies and degraded a fraction of the SP-D pool. Our results also indicated that SP-D binding sites on type II cells may differ from those on alveolar macrophages. We conclude that, in vitro, type II cells bind and recycle SP-D to lamellar bodies, but SP-D may not directly modulate surfactant uptake by type II cells.
引用
收藏
页码:L830 / L839
页数:10
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