Metabolic Pathways Involved in Cold Acclimation Identified by Integrated Analysis of Metabolites and Transcripts Regulated by DREB1A and DREB2A

被引：276

作者：

Maruyama, Kyonoshin ^{[1
]}

Takeda, Migiwa ^{[2
]}

Kidokoro, Satoshi ^{[1
,3
]}

Yamada, Kohji ^{[1
,3
]}

Sakuma, Yoh ^{[1
,3
]}

Urano, Kaoru ^{[4
]}

Fujita, Miki ^{[4
]}

Yoshiwara, Kyouko ^{[1
]}

Matsukura, Satoko ^{[1
,3
]}

Morishita, Yoshihiko ^{[2
]}

Sasaki, Ryosuke ^{[2
]}

Suzuki, Hideyuki ^{[2
]}

Saito, Kazuki ^{[2
,5
]}

Shibata, Daisuke ^{[2
]}

Shinozaki, Kazuo ^{[4
]}

Yamaguchi-Shinozaki, Kazuko ^{[1
,3
]}

机构：

[1] Japan Int Res Ctr Agr Sci, Biol Resources Div, Tsukuba, Ibaraki 3058686, Japan

[2] Kazusa DNA Res Inst, Chiba 2920818, Japan

[3] Univ Tokyo, Grad Sch Agr & Life Sci, Lab Plant Mol Physiol, Bunkyo Ku, Tokyo 1138657, Japan

[4] RIKEN, Plant Sci Ctr, Gene Discovery Res Grp, Tsukuba, Ibaraki 3050074, Japan

[5] Chiba Univ, Grad Sch Pharmaceut Sci, Dept Mol Biol & Biotechnol, Chiba 2638522, Japan

来源：

PLANT PHYSIOLOGY | 2009年 / 150卷 / 04期

关键词：

RESPONSIVE GENE-EXPRESSION; ENHANCES FREEZING TOLERANCE; CIS-ACTING ELEMENT; LOW-TEMPERATURE; ARABIDOPSIS-THALIANA; SIGNAL-TRANSDUCTION; PLANT DROUGHT; STRESS; SALT; MICROARRAY;

D O I：

10.1104/pp.109.135327

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

DREB1A/CBF3 and DREB2A are transcription factors that specifically interact with a cis-acting dehydration-responsive element (DRE), which is involved in cold-and dehydration-responsive gene expression in Arabidopsis (Arabidopsis thaliana). Overexpression of DREB1A improves stress tolerance to both freezing and dehydration in transgenic plants. In contrast, overexpression of an active form of DREB2A results in significant stress tolerance to dehydration but only slight tolerance to freezing in transgenic plants. The downstream gene products for DREB1A and DREB2A are reported to have similar putative functions, but downstream genes encoding enzymes for carbohydrate metabolism are very different between DREB1A and DREB2A. We demonstrate that under cold and dehydration conditions, the expression of many genes encoding starch-degrading enzymes, sucrose metabolism enzymes, and sugar alcohol synthases changes dynamically; consequently, many kinds of monosaccharides, disaccharides, trisaccharides, and sugar alcohols accumulate in Arabidopsis. We also show that DREB1A overexpression can cause almost the same changes in these metabolic processes and that these changes seem to improve freezing and dehydration stress tolerance in transgenic plants. In contrast, DREB2A overexpression did not increase the level of any of these metabolites in transgenic plants. Strong freezing stress tolerance of the transgenic plants overexpressing DREB1A may depend on accumulation of these metabolites.

引用

页码：1972 / 1980

页数：9