Sulfatases are unique in requiring an essential post-translational modification of a critical active-site cysteinyt or seryl residue to 3-oxoalanine usually called C alpha-formylglycine (FGly). This post-translational modification is catalyzed anaerobically by anaerobic Sulfatase Maturating Enzyme (anSME), a member of the radical AdoMet superfamily. Using a new labeled substrate, we demonstrate that anSME uses a 5'-deoxyadenosyl radical to catalyze direct H-atom abstraction from the substrate. We thus established that anSMEs are the first radical AdoMet enzymes catalyzing a post-translational modification involving C, H-atom abstraction from an active site cysteinyl or seryl residue. This mechanistic study allowed us to decipher the first steps of the mechanism of this new radical AdoMet enzyme family.
机构:
INRA, UPR 910, Unite Ecol & Physiol Syst Digestif, F-78352 Jouy En Josas, FranceINRA, UPR 910, Unite Ecol & Physiol Syst Digestif, F-78352 Jouy En Josas, France
Benjdia, Alhosna
;
Subramanian, Sowmya
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Univ Georgia, Dept Chem, Athens, GA 30602 USA
Univ Georgia, Ctr Metalloenzyme Studies, Athens, GA 30602 USAINRA, UPR 910, Unite Ecol & Physiol Syst Digestif, F-78352 Jouy En Josas, France
Subramanian, Sowmya
;
Leprince, Jerome
论文数: 0引用数: 0
h-index: 0
机构:
Univ Rouen, UA CNRS, IFRMP23, INSERM U 413, F-76821 Mont St Aignan, FranceINRA, UPR 910, Unite Ecol & Physiol Syst Digestif, F-78352 Jouy En Josas, France
Leprince, Jerome
;
Vaudry, Hubert
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h-index: 0
机构:
Univ Rouen, UA CNRS, IFRMP23, INSERM U 413, F-76821 Mont St Aignan, FranceINRA, UPR 910, Unite Ecol & Physiol Syst Digestif, F-78352 Jouy En Josas, France
Vaudry, Hubert
;
Johnson, Michael K.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Georgia, Dept Chem, Athens, GA 30602 USA
Univ Georgia, Ctr Metalloenzyme Studies, Athens, GA 30602 USAINRA, UPR 910, Unite Ecol & Physiol Syst Digestif, F-78352 Jouy En Josas, France
Johnson, Michael K.
;
Berteau, Olivier
论文数: 0引用数: 0
h-index: 0
机构:
INRA, UPR 910, Unite Ecol & Physiol Syst Digestif, F-78352 Jouy En Josas, FranceINRA, UPR 910, Unite Ecol & Physiol Syst Digestif, F-78352 Jouy En Josas, France
机构:
Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USAUniv Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Carlson, Brian L.
;
Ballister, Edward R.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA
Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USAUniv Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Ballister, Edward R.
;
Skordalakes, Emmanuel
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USAUniv Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Skordalakes, Emmanuel
;
King, David S.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USAUniv Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
King, David S.
;
Breidenbach, Mark A.
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h-index: 0
机构:
Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA
Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USAUniv Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Breidenbach, Mark A.
;
Gilmore, Sarah A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USAUniv Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Gilmore, Sarah A.
;
Berger, James M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USAUniv Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Berger, James M.
;
Bertozzi, Carolyn R.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA
Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USAUniv Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
机构:
INRA, UPR 910, Unite Ecol & Physiol Syst Digestif, F-78352 Jouy En Josas, FranceINRA, UPR 910, Unite Ecol & Physiol Syst Digestif, F-78352 Jouy En Josas, France
Benjdia, Alhosna
;
Subramanian, Sowmya
论文数: 0引用数: 0
h-index: 0
机构:
Univ Georgia, Dept Chem, Athens, GA 30602 USA
Univ Georgia, Ctr Metalloenzyme Studies, Athens, GA 30602 USAINRA, UPR 910, Unite Ecol & Physiol Syst Digestif, F-78352 Jouy En Josas, France
Subramanian, Sowmya
;
Leprince, Jerome
论文数: 0引用数: 0
h-index: 0
机构:
Univ Rouen, UA CNRS, IFRMP23, INSERM U 413, F-76821 Mont St Aignan, FranceINRA, UPR 910, Unite Ecol & Physiol Syst Digestif, F-78352 Jouy En Josas, France
Leprince, Jerome
;
Vaudry, Hubert
论文数: 0引用数: 0
h-index: 0
机构:
Univ Rouen, UA CNRS, IFRMP23, INSERM U 413, F-76821 Mont St Aignan, FranceINRA, UPR 910, Unite Ecol & Physiol Syst Digestif, F-78352 Jouy En Josas, France
Vaudry, Hubert
;
Johnson, Michael K.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Georgia, Dept Chem, Athens, GA 30602 USA
Univ Georgia, Ctr Metalloenzyme Studies, Athens, GA 30602 USAINRA, UPR 910, Unite Ecol & Physiol Syst Digestif, F-78352 Jouy En Josas, France
Johnson, Michael K.
;
Berteau, Olivier
论文数: 0引用数: 0
h-index: 0
机构:
INRA, UPR 910, Unite Ecol & Physiol Syst Digestif, F-78352 Jouy En Josas, FranceINRA, UPR 910, Unite Ecol & Physiol Syst Digestif, F-78352 Jouy En Josas, France
机构:
Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USAUniv Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Carlson, Brian L.
;
Ballister, Edward R.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA
Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USAUniv Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Ballister, Edward R.
;
Skordalakes, Emmanuel
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USAUniv Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Skordalakes, Emmanuel
;
King, David S.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USAUniv Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
King, David S.
;
Breidenbach, Mark A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA
Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USAUniv Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Breidenbach, Mark A.
;
Gilmore, Sarah A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USAUniv Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Gilmore, Sarah A.
;
Berger, James M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USAUniv Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Berger, James M.
;
Bertozzi, Carolyn R.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA
Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USAUniv Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA