Large-scale preparation of α-D-(1→4)-oligogalacturonic acids from pectic acid

被引:2
作者
Fan, HN [1 ]
Liu, MZ [1 ]
Lee, YC [1 ]
机构
[1] Johns Hopkins Univ, Dept Biol, Baltimore, MD 21218 USA
关键词
pectic acid; galacturonic acid; galabiose; galatriose; pectinase;
D O I
10.1139/V02-055
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
An efficient and inexpensive method for large-scale preparation Of alpha-D-(1-->4)-oligogalacturonic acids (oligo-GalA), up to DP 5, from pectic acid is described. Pectic acid was digested with a commercially available pectinase to yield a mixture of oligo-GalA, which was effectively separated by a combination of low-pressure - size-exclusion chromatography based on ion-exchange chromatography to obtain pure oligo-GalA of DP 2-5.
引用
收藏
页码:900 / 903
页数:4
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