Large-scale preparation of α-D-(1→4)-oligogalacturonic acids from pectic acid

被引：2

作者：

Fan, HN ^{[1
]}

Liu, MZ ^{[1
]}

Lee, YC ^{[1
]}

机构：

[1] Johns Hopkins Univ, Dept Biol, Baltimore, MD 21218 USA

来源：

CANADIAN JOURNAL OF CHEMISTRY | 2002年 / 80卷 / 08期

关键词：

pectic acid; galacturonic acid; galabiose; galatriose; pectinase;

D O I：

10.1139/V02-055

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An efficient and inexpensive method for large-scale preparation Of alpha-D-(1-->4)-oligogalacturonic acids (oligo-GalA), up to DP 5, from pectic acid is described. Pectic acid was digested with a commercially available pectinase to yield a mixture of oligo-GalA, which was effectively separated by a combination of low-pressure - size-exclusion chromatography based on ion-exchange chromatography to obtain pure oligo-GalA of DP 2-5.

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收藏

页码：900 / 903

页数：4

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