Structure, growth and cell proliferation of human osteosarcoma and malignant fibrous histiocytoma xenografts in serial transplantation in nude mice

被引：3

作者：

Brostrom, LA ^{[1
]}

Crnalic, S ^{[1
]}

Lofvenberg, R ^{[1
]}

Stenling, R ^{[1
]}

Boquist, L ^{[1
]}

机构：

[1] UMEA UNIV HOSP,DEPT PATHOL,S-90185 UMEA,SWEDEN

来源：

APMIS | 1996年 / 104卷 / 11期

关键词：

osteosarcoma; malignant fibrous histiocytoma; nude mice; cell kinetics; c-myc;

D O I：

10.1111/j.1699-0463.1996.tb04942.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Tumour specimens from one patient with osteosarcoma and one with malignant fibrous histiocytoma were transplanted in serial passages in nude mice. Structure, growth and cell kinetics of the xenografts were studied in order to assess the validity of the two tumour models. Cell proliferation was analysed using in vivo labelling with the thymidine analogue iododeoxyuridine (IdUrd) and the IdUrd labelling index (LI) was determined by immunohistochemistry. The DNA index (DI) was examined by flow cytometry. The c-myc oncoprotein expression was studied by immunohistochemistry. More intense proliferation was observed in the peripheral parts of the tumours. There was no correlation between tumour growth and cell proliferation in the two tumour groups. Stability of the tumour models was indicated by low intrapassage and interpassage variations of DI, LI, and volume doubling time, and also by retained histopathological characteristics and c-myc staining patterns of donor patients' tumours during serial transplantation.

引用

页码：775 / 783

页数：9