Experimental and theoretical bases of specific affinity, a cytoarchitecture-based formulation of nutrient collection proposed to supercede the Michaels-Menten paradigm of microbial kinetics

被引:36
作者
Button, DK [1 ]
Robertson, B
Gustafson, E
Zhao, XM
机构
[1] Univ Alaska, Inst Marine Sci, Fairbanks, AK 99775 USA
[2] Univ Alaska, Dept Chem & Biochem, Fairbanks, AK 99775 USA
关键词
D O I
10.1128/AEM.70.9.5511-5521.2004
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A theory for solute uptake by whole cells was derived with a focus on the ability of oligobacteria to sequester nutrients. It provided a general relationship that was used to obtain the kinetic constants for in situ marine populations in the presence of naturally occurring substrates. In situ affinities of 0.9 to 400 liters g of cells(-1) h(-1) found were up to 10(3) times smaller than those from a "Marinobacter arcticus" isolate, but springtime values were greatly increased by warming. Affinities of the isolate for usual polar substrates but not for hydrocarbons were diminished by ionophores. A kinetic curve or Monod plot was constructed from the best available data for cytoarchitectural components of the isolate by using the theory together with concepts and calculations from first principles. The order of effect of these components on specific affinity was membrane potential > cytoplasmic enzyme concentration > cytoplasmic enzyme affinity > permease concentration > area of the permease site > translation coefficient > porin concentration. Component balance was influential as well; a small increase in cytoplasmic enzyme concentration gave a large increase in the effect of permease concentration. The effect of permease concentration on specific affinity was large, while the effect on K-m was small. These results are in contrast to the Michaelis-Menten theory as applied by Monod that has uptake kinetics dependent on the quality of the permease molecules, with K-m as an independent measure of affinity. Calculations demonstrated that most oligobacteria in the environment must use multiple substrates simultaneously to attain sufficient energy and material for growth, a requirement consistent with communities largely comprising few species.
引用
收藏
页码:5511 / 5521
页数:11
相关论文
共 44 条
[1]  
Amdur I., 1966, Chemical Kinetics: Principles and Selected Topics
[2]   A PHYSICIST LOOKS AT BACTERIAL CHEMOTAXIS [J].
BERG, HC .
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1988, 53 :1-9
[3]   THE ANALYSIS OF RATE LIMITATION WITHIN ENZYMES - RELATIONS BETWEEN FLUX CONTROL COEFFICIENTS OF RATE CONSTANTS AND UNIDIRECTIONAL RATES, RATE CONSTANTS AND THERMODYNAMIC PARAMETERS OF SINGLE ISOLATED-ENZYMES [J].
BROWN, GC ;
COOPER, CE .
BIOCHEMICAL JOURNAL, 1994, 300 :159-164
[4]  
Button DK, 2000, ASM NEWS, V66, P510
[5]   KINETICS OF NUTRIENT-LIMITED TRANSPORT AND MICROBIAL-GROWTH [J].
BUTTON, DK .
MICROBIOLOGICAL REVIEWS, 1985, 49 (03) :270-297
[6]   Nutrient uptake by microorganisms according to kinetic parameters from theory as related to cytoarchitecture [J].
Button, DK .
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1998, 62 (03) :636-+
[7]  
Button DK, 1998, APPL ENVIRON MICROB, V64, P4467
[8]   SENSITIVE AND ACCURATE METHODOLOGY FOR MEASURING THE KINETICS OF CONCENTRATION-DEPENDENT HYDROCARBON METABOLISM RATES IN SEAWATER BY MICROBIAL COMMUNITIES [J].
BUTTON, DK ;
SCHELL, DM ;
ROBERTSON, BR .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1981, 41 (04) :936-941
[9]   KINETICS OF BACTERIAL PROCESSES IN NATURAL AQUATIC SYSTEMS BASED ON BIOMASS AS DETERMINED BY HIGH-RESOLUTION FLOW-CYTOMETRY [J].
BUTTON, DK ;
ROBERTSON, BR .
CYTOMETRY, 1989, 10 (05) :558-563
[10]   Effect of nutrient kinetics and cytoarchitecture on bacterioplankter size [J].
Button, DK ;
Robertson, B .
LIMNOLOGY AND OCEANOGRAPHY, 2000, 45 (02) :499-505