Regulation of the levels of Smad1 and Smad5 in MC3T3-E1 cells by Icariine in vitro

被引:26
作者
Zhou, Huifang [1 ,2 ]
Wang, Shuo [3 ,4 ]
Xue, Yuan [3 ]
Shi, Nianke [1 ]
机构
[1] Tianjin Med Univ, Dept Operat Surg, Tianjin 300070, Peoples R China
[2] Xiqing Hosp, Dept Anesthesia, Tianjin 300000, Peoples R China
[3] Tianjin Med Univ, Gen Hosp, Dept Orthopaed Surg, Tianjin 300052, Peoples R China
[4] 464 Hosp Peoples Liberat Army, Dept Orthopaed Surg, Tianjin 300381, Peoples R China
基金
中国国家自然科学基金;
关键词
Icariine; osteoblasts; Smads; signal transduction; HERBA-EPIMEDII; CROSS-TALK; EXPRESSION; PATHWAY; PROTEINS;
D O I
10.3892/mmr.2013.1837
中图分类号
R73 [肿瘤学];
学科分类号
100214 [肿瘤学];
摘要
The purpose of this study was to investigate the role of Icariine on the expression of Smadl and Smad5 mRNA and protein levels in MC3T3-E1 cells in vitro. MC3T3-E1 cells were cultured in the presence of different concentrations of Icariine (0, 10, 40 and 80 ng/ml). Smad1 and Smad5 mRNA levels were detected by reverse transcription-polymerase chain reaction (RT-PCR) and the expression of proteins was determined by western blotting, immunohistochemistry staining and immunofluorescence. Smad1 and Smad5 mRNA levels continuously increased in 10, 40 and 80 ng/ml of Icariine with time and the differences indicated statistical significance. Western blot analysis demonstrated that the Smad1 and Smad5 protein levels in the 10, 40 and 80 ng/ml groups were higher compared with the 0 ng/ml group at 24, 48 and 72 h, and the difference was statistically significant. Immunohistochemistry staining and immunofluorescence showed that the expression of the Smad1 and Smad5 proteins was higher in the cytoplasm and nuclei in the 10, 40 and 80 ng/ml groups compared with the 0 ng/ml group. Icariine has a direct stimulatory function on the differentiation of MC3T3-E1 osteoblastic cells in vitro, which may be mediated by increasing the production of Smad1 and Smad5 in osteoblasts.
引用
收藏
页码:590 / 594
页数:5
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