Changes in the levels of mRNAs for GH/prolactin/somatolactin family and Pit-1/GHF-1 in the pituitaries of pre-spawning chum salmon

被引:33
作者
Taniyama, S [1 ]
Kitahashi, T
Ando, H
Ban, M
Ueda, H
Urano, A
机构
[1] Hokkaido Univ, Grad Sch Sci, Div Biol Sci, Sapporo, Hokkaido 0600810, Japan
[2] Hokkaido Univ, Fac Fisheries, Dept Biol, Hakodate, Hokkaido 0418611, Japan
[3] Fisheries Agcy Japan, Natl Salmon Resources Ctr, Sapporo, Hokkaido 0620922, Japan
[4] Hokkaido Univ, Fac Fisheries, Toya Lake Stn Environm Biol, Abuta, Hokkaido 0495723, Japan
关键词
D O I
10.1677/jme.0.0230189
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Changes in the levels of pituitary mRNAs encoding GH, prolactin (PRL) and somatolactin (SL) were determined in pre-spawning chum salmon (Oncorhynchus Keta) caught at a few key points along their homing pathway in 1994 and 1995. Furthermore, we analyzed relationships between expression of pituitary-specific POU homeodomain transcription factor (Pit-1/GHF-1) and GH/PRL/SL family genes. In 1994, seawater (SW) fish and matured fresh-water (FW) fish were sequentially captured at two points along their homing pathway, the coast and the hatchery. In addition to these two points, maturing FW fish were captured at the intermediate of the two points in 1995. The levels of hormonal mRNAs were determined by a quantitative dot blot analysis using single-stranded sense DNA as the standard. Relative levels of Pit-1/GHF-1 mRNAs were estimated by Northern blot analysis. In 1994, the levels of GH/PRL/SL family mRNAs except for PRL mRNA in the male FW fish were 1.8-4 times higher than those in the SW fish. In 1995, the level of PRL mRNA was somewhat sharply elevated in the maturing FW fish soon after entry into the FW environment, while that of SL mRNA was gradually increased during upstream migration from the coast to the hatchery. The levels of 3 kb Pit-1/GHF-1 mRNA in the FW fish were higher than those in the SW fish in both 1994 and 1995. The present results indicate that expression of genes for the GH/PRL/SL family and Pit-1/GHF-1 is coincidentally enhanced in homing chum salmon. Moreover, the present study suggests that expression of the SL gene is elevated with sexual maturation, whereas that of PRL gene is elevated with osmotic change during the final stages of spawning migration.
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页码:189 / 198
页数:10
相关论文
共 28 条
[21]   ISOLATION AND CHARACTERIZATION OF SOMATOLACTIN, A NEW-PROTEIN RELATED TO GROWTH-HORMONE AND PROLACTIN FROM ATLANTIC COD (GADUS-MORHUA) PITUITARY-GLANDS [J].
RANDWEAVER, M ;
NOSO, T ;
MURAMOTO, K ;
KAWAUCHI, H .
BIOCHEMISTRY, 1991, 30 (06) :1509-1515
[22]  
RANDWEAVER M, 1993, FISH PHYSIOL BIOCHEM, V30, P1509
[23]   ISOLATION AND CHARACTERIZATION OF A NOVEL GROWTH-HORMONE CDNA FROM CHUM SALMON (ONCORHYNCHUS, KETA) [J].
SEKINE, S ;
MIZUKAMI, T ;
SAITO, A ;
KAWAUCHI, H ;
ITOH, S .
BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 1009 (02) :117-120
[24]   CLONING AND EXPRESSION OF CDNA FOR SALMON GROWTH-HORMONE IN ESCHERICHIA-COLI [J].
SEKINE, S ;
MIZUKAMI, T ;
NISHI, T ;
KUWANA, Y ;
SAITO, A ;
SATO, M ;
ITOH, S ;
KAWAUCHI, H .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (13) :4306-4310
[25]   GREATER CONSERVATION OF SOMATOLACTIN, A PRESUMED PITUITARY-HORMONE OF THE GROWTH-HORMONE PROLACTIN FAMILY, THAN OF GROWTH-HORMONE IN TELEOST FISH [J].
TAKAYAMA, Y ;
ONO, M ;
RANDWEAVER, M ;
KAWAUCHI, H .
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 1991, 83 (03) :366-374
[26]   CONTROL OF SALMON PITUITARY-HORMONE GENE-EXPRESSION [J].
XIONG, F ;
CHIN, R ;
GONG, ZY ;
SUZUKI, K ;
KITCHING, R ;
MAJUMDARSONNYLAL, S ;
ELSHOLTZ, HP ;
HEW, CL .
FISH PHYSIOLOGY AND BIOCHEMISTRY, 1993, 11 (1-6) :63-70
[27]  
YAMADA S, 1993, J BIOL CHEM, V268, P24361
[28]  
GENE