Cultivation of Fastidious Bacteria by Viability Staining and Micromanipulation in a Soil Substrate Membrane System

被引：29

作者：

Ferrari, B. C. ^{[1
]}

Gillings, M. R. ^{[2
]}

机构：

[1] Univ New S Wales, Sch Biotechnol & Biomol Sci, Randwick, NSW 2052, Australia

[2] Macquarie Univ, Dept Biol Sci, N Ryde, NSW 2019, Australia

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2009年 / 75卷 / 10期

关键词：

RATOON STUNTING DISEASE; XYLI SUBSP XYLI; FLOW-CYTOMETRY; PURE CULTURE; BACTERIOPLANKTON; ACTINOBACTERIA; MICROORGANISMS; HYBRIDIZATION; MICROBIOLOGY; DIVERSE;

D O I：

10.1128/AEM.02407-08

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Soil substrate membrane systems allow for microcultivation of fastidious soil bacteria as mixed microbial communities. We isolated established microcolonies from these membranes by using fluorescence viability staining and micromanipulation. This approach facilitated the recovery of diverse, novel isolates, including the recalcitrant bacterium Leifsonia xyli, a plant pathogen that has never been isolated outside the host.

引用

页码：3352 / 3354

页数：3

共 32 条

[31] Genetic uniformity of international isolates of Leifsonia xyli subsp xyli, causal agent of ratoon stunting disease of sugarcane [J].

Young, A. J. ;

Petrasovits, L. A. ;

Croft, B. J. ;

Gillings, M. ;

Brumbley, S. M. .

AUSTRALASIAN PLANT PATHOLOGY, 2006, 35 (05) :503-511

[32] Cultivating the uncultured [J].

Zengler, K ;

Toledo, G ;

Rappé, M ;

Elkins, J ;

Mathur, EJ ;

Short, JM ;

Keller, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (24) :15681-15686

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