Attachment of 3T3 and MDBK cells onto poly(EGDMA/HEMA) based microbeads and their biologically modified forms

被引:4
作者
Ayhan, H [1 ]
Gürhan, I
Piskin, E
机构
[1] Hacettepe Univ, Dept Chem Engn, Div Bioengn, TR-06532 Ankara, Turkey
[2] TUBITAK, Ctr Excellence Polymer Biomat, Ankara, Turkey
[3] Foot & Mouth Dis Inst, Ankara, Turkey
来源
ARTIFICIAL CELLS BLOOD SUBSTITUTES AND IMMOBILIZATION BIOTECHNOLOGY | 2000年 / 28卷 / 02期
关键词
poly(EGDMA/HEMA) based microbeads; biological modification; fibronectine; collagen; cell attachment; 3T3 and MDBK cell lines; mathematical simulation of attachment kinetics;
D O I
10.3109/10731190009118578
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Poly(EGDMA/HEMA) based microbeads were prepared by suspension polymerization. A comonomer, i.e., 2-hydroxyethylmetacrylate (HEMA) was included in the recipe in order to have functional hydroxyl groups on the microbead surfaces. Toluene was used in the polymerization formulations to introduce porosity into the matrix. Hydroxyl groups were first oxidized with NaIO4, and then two biological molecules, namely collagen and fibronectin were immobilized by using glutaraldehyde. A spacer-arm, i.e., hexamethylene diamine, was also used in some cases. More protein molecules were immobilized onto more swellable microbeads using spacer-arm. Higher amounts of collagen were immobilized, more than fibronectin immobilization. Attachment of two cell lines (i.e., 3T3 and MDBK cell lines) on these microbeads with a wide variety of surface properties was studied in vino culture media. Attachments of both cells even onto the plain microbeads were significant. More cells did attach to more swellable microbeads. Introducing both fibronectin and collagen onto the microbeads caused significant increase in the cell attachment. More cells attached to the microbeads carrying fibronectin covalently attached onto the microbeads through the spacer-arm molecules. Fibronectine was better than collagen for high attachment values. The mathematical model proposed successfully simulated attachment kinetics.
引用
收藏
页码:155 / 171
页数:17
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