Intermediate conductance Ca2+-activated potassium channel (KCa3.1) in the inner mitochondrial membrane of human colon cancer cells

被引：93

作者：

De Marchi, Umberto ^{[2
]}

Sassi, Nicola ^{[2
]}

Fioretti, Bernard ^{[3
]}

Catacuzzeno, Luigi ^{[3
]}

Cereghetti, Grazia M. ^{[4
]}

Szabo, Ildiko ^{[5
]}

Zoratti, Mario ^{[1
,2
]}

机构：

[1] CNR Inst Neurosci, Dept Biomed Sci, I-35121 Padua, Italy

[2] Univ Padua, Dept Biomed Sci, Padua, Italy

[3] Univ Perugia, Dept Cellular & Environm Biol, I-06100 Perugia, Italy

[4] Venetian Inst Mol Med, Padua, Italy

[5] Univ Padua, Dept Biol, Padua, Italy

来源：

CELL CALCIUM | 2009年 / 45卷 / 05期

关键词：

Mitochondria; Patch-clamp; Inner membrane; IK(Ca); HCT116; SENSITIVE K+ CHANNEL; PERMEABILITY TRANSITION; ANION CHANNEL; MICE LACKING; IN-VITRO; CALCIUM; LOCALIZATION; CALMODULIN; MODULATION; EXPRESSION;

D O I：

10.1016/j.ceca.2009.03.014

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Patch-clamping mitoplasts isolated from human colon carcinoma 116 cells has allowed the identification and characterization of the intermediate conductance Ca2+-activated K+-selective channel K(Ca)3.1, previously studied only in the plasma membrane of various cell types. Its identity has been established by its biophysical and pharmacological properties. Its localisation in the inner membrane of mitochondria is indicated by Western blots of subcellular fractions, by recording of its activity in mitochondria made fluorescent by a mitochondria-targeted fluorescent protein and by the co-presence of channels considered to be markers of the inner membrane. Moderate increases of mitochondrial matrix [Ca2+] will cause mtK(Ca)3.1 opening, thus linking inner membrane K+ permeability and transmembrane potential to Ca2+ signalling. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：509 / 516

页数：8

共 82 条

[1] KCa3.1 potassium channels are critical for cAMP-dependent chloride secretion and cyst growth in autosomal-dominant polycystic kidney disease [J].