A polyamidoamine dendrimer-capped mesoporous silica nanosphere-based gene transfection reagent

被引：728

作者：

Radu, DR

Lai, CY

Jeftinija, K

Rowe, EW

Jeftinija, S

Lin, VSY ^{[1
]}

机构：

[1] Iowa State Univ, Dept Chem, Ames, IA 50011 USA

[2] Iowa State Univ, Dept Biomed Sci, Ames, IA 50011 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 41期

关键词：

D O I：

10.1021/ja046275m

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We synthesized a MCM-41-type mesoporous silica nanosphere (MSN)-based gene transfection system, where second generation (G2) polyamidoamines (PAMAMs) were covalently attached to the surface of MSN. The G2-PAMAM-capped MSN material (G2-MSN) was used to complex with a plasmid DNA (pEGFP-C1) that encodes for an enhanced green fluorescence protein. The gene transfection efficacy, uptake mechanism, and biocompatibility of the G2-MSN system with various cell types, such as neural glia (astrocytes), human cervical cancer (HeLa), and Chinese hamster ovarian (CHO) cells, were investigated. The mesoporous structure of the MSN material allows membrane-impermeable molecules, such as pharmaceutical drugs and fluorescent dyes, to be encapsulated inside the MSN channels. The system renders the possibility to serve as a universal transmembrane carrier for intracellular drug delivery and imaging applications. Copyright © 2004 American Chemical Society.

引用

页码：13216 / 13217

页数：2

共 9 条

[1]

DENNIG J, 2002, REV MOL BIOTECHNOL, V90, P339, DOI DOI 10.1016/S1389-0352(01)00066-6

[2] Poly(amidoamine) (PAMAM) dendrimers: from biomimicry to drug delivery and biomedical applications [J].