Preparation and application of functionalized nanoparticles of CdSe capped with 11-mercaptoundecanoic acid as a fluorescence probe

被引：24

作者：

Zhong, Ping

Yu, Ying ^{[1
]}

Wu, Jianzhong

Lai, Yan

Chen, Bo

Long, Zhaoyang

Liang, Chunsui

机构：

[1] S China Normal Univ, Sch Chem & Environm, Guangzhou 510006, Peoples R China

[2] Ctr Dis Control & Prevent Guangdong Prov, Guangzhou 510300, Peoples R China

来源：

TALANTA | 2006年 / 70卷 / 04期

关键词：

nanoparticles; nanocrystals; 11-mercaptoundecanoic acid; fluorescence probe; lysozyme;

D O I：

10.1016/j.talanta.2006.03.015

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new fluorescence probe, CdSe nanocrystal has been prepared and modified with 11-mercaptoundecanoic acid [HS-(CH2)(10)-COOH]. The functionalized nanoparticles were characterized using transmission electron microscopy (TEM), ultraviolet-visible (UV-vis) spectroscopy and photoluminescence (PL) spectroscopy. The results demonstrate that CdSe is dispersed homogeneously in aqueous solution and well protected from the environmental oxygen. They can be used as a new fluorescence probe for lysozyme, which was simple, rapid and specific. Under the optimum condition, the response is linearly proportional to the amount of lysozyme from 0.20 to 26.0 mu g ml(-1), and the limit of detection is 0.115 mu g ml(-1). The proposed method has been applied to the determination of lysozyme in egg white, with the recovery of 96-105%. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：902 / 906

页数：5

共 32 条

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