Development and Application of an Electrochemical Plate Coupled with Immunomagnetic Beads (ELIME) Array for Salmonella enterica Detection in Meat Samples

被引：31

作者：

Delibato, Elisabetta ^{[2
]}

Volpe, Giulia ^{[1
]}

Romanazzo, Daniela ^{[1
]}

De Medici, Dario ^{[2
]}

Toti, Laura ^{[2
]}

Moscone, Danila ^{[1
]}

Palleschi, Giuseppe ^{[1
]}

机构：

[1] Univ Roma Tor Vergata, Dipartimento Sci & Tecnol Chim, I-00133 Rome, Italy

[2] Ist Super Sanita, Dipartimento Sanita Pubbl Vet & Sicurezza Aliment, I-00161 Rome, Italy

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2009年 / 57卷 / 16期

关键词：

Salmonella; meat samples; ELIME array; immunomagnetic beads; REAL-TIME PCR; RAPID DETECTION; FOODS; IDENTIFICATION; ENTERITIDIS; BIOSENSOR; PRODUCTS; ELISA; SPP;

D O I：

10.1021/jf901181m

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Salmonella is one of the main organisms causing outbreaks of foodborne illness, and meat is one of the major vehicles of salmonellosis throughout the world. A novel analytical immunosensor array, based on a 96-well electrochemical plate coupled with immunomagnetic beads (ELIME array), is proposed for the detection of Salmonella in meat samples. After an optimization study, using Salmonella enterica serotype Enteritidis as reference antigen, the ability of the method to interact with a large number of Salmonella serovars commonly present in food was evaluated. The assay was then used to analyze samples of pork, chicken, beef, and turkey experimentally inoculated with Salmonella as well as real samples. The results were compared with those from the International Standard of Organization (ISO) culture method. The comparison showed that the ELIME array is able to detect a low number of Salmonella cells (1-10 CFU per 25 g) after only 6 h of incubation in a pre-enrichment broth. The investigation revealed a very good agreement between culture and ELIME array methods for meat samples, reducing the time for performing the analysis and obtaining the results quickly.

引用

页码：7200 / 7204

页数：5

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