A novel glycosyltransferase with a polyglutamine repeat;: a new candidate for GD1α synthase (ST6GalNAc V)

被引：37

作者：

Ikehara, Y ^{[1
]}

Shimizu, N

Kono, M

Nishihara, S

Nakanishi, H

Kitamura, T

Narimatsu, H

Tsuji, S

Tatematsu, M

机构：

[1] Aichi Canc Ctr, Res Inst, Div Pathol, Chikusa Ku, Nagoya, Aichi 4640021, Japan

[2] Univ Tokyo, Postgrad Med Sch, Dept Gastrointestinal Surg, Bunkyo Ku, Tokyo 1128688, Japan

[3] RIKEN, Inst Phys & Chem Res, Frontier Res Program, Dept Glyco Mol Biol, Wako, Saitama 35101, Japan

[4] Soka Univ, Inst Life Sci, Div Cell Biol, Tokyo 1928857, Japan

[5] Aichi Gakuin Univ, Sch Dent, Dept Oral & Maxillofacial Surg 1, Chikusa Ku, Nagoya, Aichi 4640021, Japan

来源：

FEBS LETTERS | 1999年 / 463卷 / 1-2期

关键词：

GalNAc alpha 2,6-sialyltransferase; ST6GalNAc V; GD1 alpha synthase; polyglutamine repeat; central nervous system;

D O I：

10.1016/S0014-5793(99)01605-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The fifth type GalNAc alpha 2,6-sialyltransferase (mST6GalNAc V) was cloned from a mouse brain cDNA library. mST6GalNAc V exhibited type II transmembrane topology containing a polyglutamine repeat, which showed 42.6% and 44.8% identity to mouse ST6GalNAc III and IV, respectively. Northern blot analysis revealed that the mST6GalNAc V gene was specifically expressed in forebrain and cerebellum. mST6GalNAc V exhibited GD1 alpha synthetic activity from GM1b the same as mST6GalNAc III and IV. The activity ratio of GM1b toward fetuin and the expression pattern were completely different among the three ST6GalNAcs, Interestingly, the polyglutamine repeat number was different from that of inbred mice, We report the first glycosyltransferase with a polymorphic polyglutamine repeat. (C) 1999 Federation of European Biochemical Societies.

引用

页码：92 / 96

页数：5

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