Genetic relationship in southern African Crimean-Congo haemorrhagic fever virus isolates: evidence for occurrence of reassortment

被引：42

作者：

Burt, F. J. ^{[1
]}

Paweska, J. T. ^{[3
]}

Ashkettle, B. ^{[2
]}

Swanepoel, R. ^{[3
]}

机构：

[1] Natl Hlth Lab Serv Univ, Dept Med Virol, Bloemfontein, South Africa

[2] Univ Free State, Fac Hlth Sci, Dept Med Virol, Bloemfontein, South Africa

[3] Natl Inst Communicable Dis, Special Pathogens Unit, Johannesburg, South Africa

来源：

EPIDEMIOLOGY AND INFECTION | 2009年 / 137卷 / 09期

关键词：

CCHF; genetic reassortment; POLYMERASE-CHAIN-REACTION; M-RNA SEGMENT; STRAINS; EPIDEMIOLOGY; SEQUENCES; DIAGNOSIS; RUSSIA;

D O I：

10.1017/S0950268808001878

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Crimean-Congo haemorrhagic fever (CCHF) is a tick-borne viral zoonosis widely distributed in Africa, Asia and eastern Europe. Reassortment of CCHF genome segments has been shown to occur in nature. We therefore investigated the genetic relationship of southern African isolates using partial sequence data for each RNA segment, S, M and L, and comparing the tree topologies constructed using a neighbour joining method. A total of 21 southern African isolates were studied. The incongruencies which were identified in S, M and L sequence datasets involved group switching implying reassortment for 15 isolates. A higher fatality rate occurred in patients infected with isolates which had apparently acquired M segments from a group in which predominantly Asian strains are usually found. This suggests that reassortment may affect the pathogenicity of the virus.

引用

页码：1302 / 1308

页数：7

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