Expression of a bacterial endoglucanase gene in tobacco increases digestibility of its cell wall fibers

被引:18
作者
Kawazu, T
Sun, JL
Shibata, M
Kimura, T
Sakka, K
Ohmiya, K
机构
[1] Oji Paper Co Ltd, Forestry Res Inst, Kameyama, Mie 5190212, Japan
[2] Mie Univ, Fac Bioresources, Tsu, Mie 5148507, Japan
关键词
cell wall; endoglucanase; Ruminococcus albus; transgenic tobacco;
D O I
10.1016/S1389-1723(99)80220-5
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Transgenic tobacco plants exhibiting exogenous cellulase activity were produced by introduction of a truncated cellulase (t-EgI) gene from Ruminococcus albus. They accumulated about 0.1-0.5% t-EgI of the total soluble proteins in the cytoplasm. They were normal in morphology and growth. t-EgI expressed in the transgenic plants degraded their own cell wall materials after the cells were disrupted. The plants expressing the t-egI gene could be used as "cellulase-encapsulating plants" for livestock and food industries.
引用
收藏
页码:421 / 425
页数:5
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