CuZn-superoxide dismutase in human thymus: immunocytochemical localisation and secretion in thymus-derived epithelial and fibroblast cell lines

被引：34

作者：

Cimini, V

Ruggiero, G

Buonomo, T

Seru, R

Sciorio, S

Zanzi, C

Santangelo, F

Mondola, P

机构：

[1] Univ Naples Federico II, Dipartimento Sci Biomorfol & Funzionali, Fac Med & Chirurg, I-80131 Naples, Italy

[2] Univ Naples, Fac Med & Chirurg, Dipartimento Biol & Patol Cellulare & Mol, I-80131 Naples, Italy

[3] Univ Naples Federico II, Dipartimento Neurosci & Sci Comunicazione Interum, I-80131 Naples, Italy

来源：

HISTOCHEMISTRY AND CELL BIOLOGY | 2002年 / 118卷 / 02期

关键词：

superoxide dismutase (SOD); thymus; immunocytochernistry; antioxidant enzymes; flow cytometry;

D O I：

10.1007/s00418-002-0429-8

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

CuZn-superoxide dismutase (SOD) plays a key role in mediating the cellular response to oxidative stress. Its expression in normal thymus was investigated by immunohistochemical techniques and CD3-, CD68- and cytokeratin-specific staining, in order to identify thymocytes, macrophages and epithelial components. Immunocytochemical studies showed an overall CuZn-SOD thymic distribution with a prevailing concentration within thymic medulla. The analysis of CuZn-SOD release by thymus-derived epithelial and fibroblast cell lines showed the ability of both cell lines to release the anti-oxidant enzyme, especially in the presence of stress conditions as represented by serum and nutrient deprivation. These data suggest that CuZn-SOD could be a relevant antioxidant paracrine molecule in human thymus.

引用

页码：163 / 169

页数：7

共 26 条

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