CuZn-superoxide dismutase in human thymus: immunocytochemical localisation and secretion in thymus-derived epithelial and fibroblast cell lines

被引:34
作者
Cimini, V
Ruggiero, G
Buonomo, T
Seru, R
Sciorio, S
Zanzi, C
Santangelo, F
Mondola, P
机构
[1] Univ Naples Federico II, Dipartimento Sci Biomorfol & Funzionali, Fac Med & Chirurg, I-80131 Naples, Italy
[2] Univ Naples, Fac Med & Chirurg, Dipartimento Biol & Patol Cellulare & Mol, I-80131 Naples, Italy
[3] Univ Naples Federico II, Dipartimento Neurosci & Sci Comunicazione Interum, I-80131 Naples, Italy
关键词
superoxide dismutase (SOD); thymus; immunocytochernistry; antioxidant enzymes; flow cytometry;
D O I
10.1007/s00418-002-0429-8
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
CuZn-superoxide dismutase (SOD) plays a key role in mediating the cellular response to oxidative stress. Its expression in normal thymus was investigated by immunohistochemical techniques and CD3-, CD68- and cytokeratin-specific staining, in order to identify thymocytes, macrophages and epithelial components. Immunocytochemical studies showed an overall CuZn-SOD thymic distribution with a prevailing concentration within thymic medulla. The analysis of CuZn-SOD release by thymus-derived epithelial and fibroblast cell lines showed the ability of both cell lines to release the anti-oxidant enzyme, especially in the presence of stress conditions as represented by serum and nutrient deprivation. These data suggest that CuZn-SOD could be a relevant antioxidant paracrine molecule in human thymus.
引用
收藏
页码:163 / 169
页数:7
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