Secretion and increase of intracellular CuZn superoxide dismutase content in human neuroblastoma SK-N-BE cells subjected to oxidative stress

被引：38

作者：

Mondola, P ^{[1
]}

Annella, T ^{[1
]}

Serù, R ^{[1
]}

Santangelo, F ^{[1
]}

Iossa, S ^{[1
]}

Gioielli, A ^{[1
]}

Santillo, M ^{[1
]}

机构：

[1] Univ Naples, Dipartimento Neurosci & Communicaz Interumana, Sez Fisiol, I-80131 Naples, Italy

来源：

BRAIN RESEARCH BULLETIN | 1998年 / 45卷 / 05期

关键词：

CuZn superoxide dismutase; neuroblastoma cells; oxidative stress; oxygen free radicals; lipid peroxides;

D O I：

10.1016/S0361-9230(97)00438-3

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

CuZn superoxide dismutase (SOD) secretion was detected in media of [S-35]cysteine-labeled human neuroblastoma SK-N-BE cells precipitated with antihuman CuZn SOD antibodies. The ability of Fe2+/ascorbate oxidative stress to induce CuZn SOD in SK-N-BE cells was evaluated by Western blot analysis. The results showed that, like human hepatocarcinoma cells and human fibroblasts, SK-N-BE cells secrete CuZn SOD. In addition, the CuZn SOD concentration was higher in cells subjected to oxidative stress than in unstressed cells. The secretion of CuZn SOD and the ability of Fe2+/ascorbate to increase its protein content in SK-N-BE cells indicates that this enzyme protects the brain from damage induced by oxidative stress. (C) 1998 Elsevier Science Inc.

引用

页码：517 / 520

页数：4

共 27 条

[1] ASPECTS OF THE STRUCTURE, FUNCTION, AND APPLICATIONS OF SUPEROXIDE-DISMUTASE [J].

BANNISTER, JV ;

BANNISTER, WH ;

ROTILIO, G .

CRC CRITICAL REVIEWS IN BIOCHEMISTRY, 1987, 22 (02) :111-180

[2]

BASS DA, 1983, J IMMUNOL, V130, P1910

[3] FREE-RADICAL SCAVENGING SYSTEMS OF RAT ASTROGLIAL CELLS IN PRIMARY CULTURE - EFFECTS OF ANOXIA AND DRUG-TREATMENT [J].

COPIN, JC ;

LEDIG, M ;

THOLEY, G .

NEUROCHEMICAL RESEARCH, 1992, 17 (07) :677-682

[4] CLONING AND SEQUENCING OF A RAT CUZN SUPEROXIDE-DISMUTASE CDNA - CORRELATION BETWEEN CUZN SUPEROXIDE-DISMUTASE MESSENGER-RNA LEVEL AND ENZYME-ACTIVITY IN RAT AND MOUSE-TISSUES [J].

DELABAR, JM ;

NICOLE, A ;

DAURIOL, L ;

JACOB, Y ;

MEUNIERROTIVAL, M ;

GALIBERT, F ;

SINET, PM ;

JEROME, H .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1987, 166 (01) :181-187

[5]

ESTERBAUER H, 1990, METHOD ENZYMOL, V186, P407

[6] HUMAN GAMMA-GAMMA-ENOLASE - 2-SITE IMMUNORADIOMETRIC ASSAY WITH A SINGLE MONOCLONAL-ANTIBODY [J].

FEDERSPPIEL, BSS ;

CRAS, P ;

GHEUENS, J ;

ANDRIES, D ;

LOWENTHAL, A .

JOURNAL OF NEUROCHEMISTRY, 1987, 48 (01) :22-28

[7] ANTIOXIDANT ENZYMATIC SYSTEMS IN NEURONAL AND GLIAL CELL-ENRICHED FRACTIONS OF RAT-BRAIN DURING AGING [J].

GEREMIA, E ;

BARATTA, D ;

ZAFARANA, S ;

GIORDANO, R ;

PINIZZOTTO, MR ;

LAROSA, MG ;

GAROZZO, A .

NEUROCHEMICAL RESEARCH, 1990, 15 (07) :719-723

[8]

Halliwell B., 1989, Free radicals in biology and medicine, V2nd

[9] REGULATION OF ANTIOXIDANT ENZYMES [J].

HARRIS, ED .

FASEB JOURNAL, 1992, 6 (09) :2675-2683

[10] OXYGEN FREE-RADICALS AND BRAIN-DYSFUNCTION [J].

JESBERGER, JA ;

RICHARDSON, JS .

INTERNATIONAL JOURNAL OF NEUROSCIENCE, 1991, 57 (1-2) :1-17

← 1 2 3 →