Neudesin, an extracellular heme-binding protein, suppresses adipogenesis in 3T3-L1 cells via the MAPK cascade

被引：47

作者：

Kimura, Ikuo ^{[1
]}

Konishi, Morichika ^{[2
]}

Asaki, Toshiyuki

Furukawa, Naoko ^{[1
]}

Ukai, Kiyotaka ^{[1
]}

Mori, Masahiro ^{[1
]}

Hirasawa, Akira ^{[3
]}

Tsujimoto, Gozoh ^{[3
]}

Ohta, Mitsuhiro ^{[4
]}

Itoh, Nobuyuki ^{[2
]}

Fujimoto, Masafumi ^{[1
]}

机构：

[1] Chiba Inst Sci, Lab Appl Pharmacol, Fac Pharm, Chiba 2880025, Japan

[2] Kyoto Univ, Dept Biochem Genet, Grad Sch Pharmaceut Sci, Sakyo Ku, Kyoto 6068501, Japan

[3] Kyoto Univ, Dept Genom Drug Discovery Sci, Grad Sch Pharmaceut Sci, Sakyo Ku, Kyoto 6068501, Japan

[4] Kobe Pharmaceut Univ, Dept Med Biochem, Kobe, Hyogo 6588558, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 381卷 / 01期

关键词：

Adipocytes; Adipogenesis; 3T3-L1; Neudesin; Heme-binding protein; Obesity; Heme; MAPK; ACTIVATED-RECEPTOR-GAMMA; ADIPOCYTE DIFFERENTIATION; NEUROTROPHIC ACTIVITY; GENE-EXPRESSION; PPAR-GAMMA; PROLIFERATION; PREADIPOCYTES; PATHWAY; KINASE; FGF10;

D O I：

10.1016/j.bbrc.2009.02.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Adult mice abundantly express neudesin, an extracellular heme-binding protein with neurotrophic activity, in white adipose tissues. At the early stage of adipocyte differentiation during adipogenesis, however, the expression of neudesin decreased transiently. Neudesin-hemin significantly suppressed adipogenesis in 3T3-L1 cells. The knockdown of neudesin by RNA interference markedly promoted adipogenesis in 3T3-L1 cells and decreased MAPK activation during adipocyte differentiation. The addition or knockdown of neudesin affected the expression of C/EBP alpha and PPAR gamma but not of C/EBP beta. These findings suggest that neudesin plays a critical role in the early stage of adipocyte differentiation in which C/EBP beta induces PPAR gamma and C/EBP alpha expressions, by controlling the MAPK pathway. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：75 / 80

页数：6

共 23 条

[1]

Roles of fibroblast growth factor 10 (Fgf10) in adipogenesis in vivo [J].