Sphingosine 1-phosphate stimulates cell migration through a Gi-coupled cell surface receptor -: Potential involvement in angiogenesis

被引:337
作者
Wang, F
Van Brocklyn, JR
Hobson, JP
Movafagh, S
Zukowska-Grojec, Z
Milstien, S
Spiegel, S
机构
[1] Georgetown Univ, Med Ctr, Dept Biochem & Mol Biol, Washington, DC 20007 USA
[2] Georgetown Univ, Med Ctr, Dept Physiol & Biophys, Washington, DC 20007 USA
[3] NIMH, Lab Cellular & Mol Regulat, NIH, Bethesda, MD 20892 USA
关键词
D O I
10.1074/jbc.274.50.35343
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Sphingosine l-phosphate (SPP) has been shown to inhibit chemotaxis of a variety of cells, in some cases through intracellular actions, while in others through receptor-mediated effects. Surprisingly, we found that low concentrations of SPP (10-100 nm) increased chemotaxis of HEK293 cells overexpressing the G protein-coupled SPP receptor EDG-1. In agreement with previous findings in human breast cancer cells (Wang, F., Nohara, K., Olivera, O., Thompson, E. W., and Spiegel, S. (1999) Exp. Cell Res. 247, 17-28), SPP, at micromolar concentrations, inhibited chemotaxis of both vector- and EDG-1-overexpressing HEK293 cells. Nanomolar concentrations of SPP also induced a marked increase in chemotaxis of human umbilical vein endothelial cells (HUVEC) and bovine aortic endothelial cells (BAEC), which express the SPP receptors EDG-1 and EDG-3, while higher concentrations of SPP were less effective. Treatment with pertussis toxin, which ADP-ribosylates and inactivates G(i)-coupled receptors, blocked SPP-induced chemotaxis. Checkerboard analysis indicated that SPP stimulates both chemotaxis and chemokinesis. Taken together, these data suggest that SPP stimulates cell migration by binding to EDG-1. Similar to SPP, sph-inganine 1-phosphate (dihydro-SPP), which also binds to this family of SPP receptors, enhanced chemotaxis; whereas, another structurally related lysophospholipid, lysophosphatidic acid, did not compete with SPP for binding nor did it have significant effects on chemotaxis of endothelial cells. Furthermore, SPP increased proliferation of HUVEC and BAEC in a pertussis toxin-sensitive manner. SPP and dihydro-SPP also stimulated tube formation of BAEC grown on collagen gels (in vitro angiogenesis), and potentiated tube formation induced by basic fibroblast growth factor. Pertussis toxin treatment blocked SPP-, but not bFGF-stimulated in vitro angiogenesis. Our results suggest that SPP may play a role in angiogenesis through binding to endothelial cell Gi-coupled SPP receptors.
引用
收藏
页码:35343 / 35350
页数:8
相关论文
共 81 条
  • [51] INTERNALIZATION OF BASIC FIBROBLAST GROWTH-FACTOR (BFGF) IN CULTURED ENDOTHELIAL-CELLS - ROLE OF THE LOW AFFINITY HEPARIN-LIKE BFGF RECEPTORS
    RUSNATI, M
    URBINATI, C
    PRESTA, M
    [J]. JOURNAL OF CELLULAR PHYSIOLOGY, 1993, 154 (01) : 152 - 161
  • [52] SPHINGOSINE 1-PHOSPHATE, A SPECIFIC ENDOGENOUS SIGNALING MOLECULE CONTROLLING CELL MOTILITY AND TUMOR-CELL INVASIVENESS
    SADAHIRA, Y
    RUAN, FQ
    HAKOMORI, SI
    IGARASHI, Y
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (20) : 9686 - 9690
  • [53] SPHINGOSINE-1-PHOSPHATE INHIBITS EXTRACELLULAR-MATRIX PROTEIN-INDUCED HAPTOTACTIC MOTILITY BUT NOT ADHESION OF B16 MOUSE MELANOMA-CELLS
    SADAHIRA, Y
    ZHENG, MZ
    RUAN, FQ
    HAKOMORI, SI
    IGARASHI, Y
    [J]. FEBS LETTERS, 1994, 340 (1-2): : 99 - 103
  • [54] Synergism among lysophosphatidic acid, β1A integrins, and epidermal growth factor or platelet-derived growth factor in mediation of cell migration
    Sakai, T
    de la Pena, JM
    Mosher, DF
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (22) : 15480 - 15486
  • [55] Restoration of β1A integrins is required for lysophosphatidic acid-induced migration of β1-null mouse fibroblastic cells
    Sakai, T
    Peyruchaud, O
    Fässler, R
    Mosher, DF
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (31) : 19378 - 19382
  • [56] Activation of phospholipase C-Ca2+ system by sphingosine 1-phosphate in CHO cells transfected with Edg-3, a putative lipid receptor
    Sato, K
    Kon, J
    Tomura, H
    Osada, M
    Murata, N
    Kuwabara, A
    Watanabe, T
    Ohta, H
    Ui, M
    Okajima, F
    [J]. FEBS LETTERS, 1999, 443 (01) : 25 - 30
  • [57] Possible involvement of cell surface receptors in sphingosine 1-phosphate-induced activation of extracellular signal-regulated kinase in C6 glioma cells
    Sato, K
    Tomura, H
    Igarashi, Y
    Ui, M
    Okajima, F
    [J]. MOLECULAR PHARMACOLOGY, 1999, 55 (01) : 126 - 133
  • [58] FIBROBLAST GROWTH-FACTORS - AT THE HEART OF ANGIOGENESIS
    SLAVIN, J
    [J]. CELL BIOLOGY INTERNATIONAL, 1995, 19 (05) : 431 - 444
  • [59] Sphingosine-1-phosphate in cell growth and cell death
    Spiegel, S
    Cuvillier, O
    Edsall, LC
    Kohama, T
    Menzeleev, R
    Olah, Z
    Olivera, A
    Pirianov, G
    Thomas, DM
    Tu, ZX
    Van Brocklyn, JR
    Wang, F
    [J]. SPHINGOLIPIDS AS SIGNALING MODULATORS IN THE NERVOUS SYSTEM, 1998, 845 : 11 - 18
  • [60] Invasion of T-lymphoma cells: cooperation between Rho family GTPases and lysophospholipid receptor signaling
    Stam, JC
    Michiels, F
    van der Kammen, RA
    Moolenaar, WH
    Collard, JG
    [J]. EMBO JOURNAL, 1998, 17 (14) : 4066 - 4074