Identification of a Pentaketide Stilbene Produced by a Type III Polyketide Synthase from Pinus sylvestris and Characterisation of Free Coenzyme A Intermediates

被引：11

作者：

Li, Tsung-Lin ^{[1
]}

Spiteller, Dieter ^{[2
]}

Spencer, Jonathan B. ^{[3
]}

机构：

[1] Acad Sinica, Genom Res Ctr, Taipei 115, Taiwan

[2] Max Planck Inst Chem Ecol, D-07745 Jena, Germany

[3] Univ Cambridge, Univ Chem Lab, Cambridge CB2 1EW, England

来源：

CHEMBIOCHEM | 2009年 / 10卷 / 05期

基金：

英国生物技术与生命科学研究理事会;

关键词：

biosynthesis; malonylresveratrol; polyketide intermediates; polyketides; stilbene synthase; CHALCONE SYNTHASE; BIOSYNTHESIS; PATHWAY; COA;

D O I：

10.1002/cbic.200800840

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The stilbene synthase (STS) from Scots pine (Pinus sylvestris), which is a type III polyketide synthase, produces the well known tetraketide resveratrol from coumaroyl-CoA and three molecules of malonyl-CoA. The same stilbene synthase, however, also generates the previously unknown pentaketide 2-malonylresveratrol from coumaroyl-CoA and four molecules of malonyl-CoA; this indicates that the enzyme does not precisely control the number of condensations leading to diverse products. Tetraketide- and pentaketide-CoA intermediates of the STS were identified by LC-MS/MS; this suggests that CoA-bound polyketide intermediates are involved in the product formation of type III polyketide synthases.

引用

页码：896 / 901

页数：6

共 24 条

[1] Engineered biosynthesis of plant polyketides: Chain length control in an octaketide-producing plant type III polyketide synthase [J].