hPOC5 is a centrin-binding protein required for assembly of full-length centrioles

被引：137

作者：

Azimzadeh, Juliette ^{[1
]}

Hergert, Polla ^{[2
]}

Delouvee, Annie ^{[1
]}

Euteneuer, Ursula ^{[3
]}

Formstecher, Etienne ^{[4
]}

Khodjakov, Alexey ^{[2
]}

Bornens, Michel ^{[1
]}

机构：

[1] Ctr Natl Rech Sci, Inst Curie, Unite Mixte Rech 144, F-75248 Paris 05, France

[2] New York State Dept Hlth, Wadsworth Ctr, Albany, NY 12201 USA

[3] Univ Munich, Adolf Butenandt Inst, D-80336 Munich, Germany

[4] Hybrigenics, F-75014 Paris, France

来源：

JOURNAL OF CELL BIOLOGY | 2009年 / 185卷 / 01期

关键词：

POLE BODY DUPLICATION; NUCLEOTIDE EXCISION-REPAIR; CELL-CYCLE PROGRESSION; BASAL-BODY; CHLAMYDOMONAS-REINHARDTII; VERTEBRATE CELLS; ELECTRON-MICROSCOPY; MITOTIC CELLS; FISSION YEAST; CENTROSOME;

D O I：

10.1083/jcb.200808082

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Centrin has been shown to be involved in centrosome biogenesis in a variety of eukaryotes. In this study, we characterize hPOC5, a conserved centrin-binding protein that contains Sfi1p-like repeats. hPOC5 is localized, like centrin, in the distal portion of human centrioles. hPOC5 recruitment to procentrioles occurs during G2/M, a process that continues up to the full maturation of the centriole during the next cell cycle and is correlated with hyperphosphorylation of the protein. In the absence of hPOC5, RPE1 cells arrest in G1 phase, whereas HeLa cells show an extended S phase followed by cell death. We show that hPOC5 is not required for the initiation of procentriole assembly but is essential for building the distal half of centrioles. Interestingly, the hPOC5 family reveals an evolutionary divergence between vertebrates and organisms like Drosophila melanogaster or Caenorhabditis elegans, in which the loss of hPOC5 may correlate with the conspicuous differences in centriolar structure.

引用

页码：101 / 114

页数：14