The ubiquitin 26S proteasome proteolytic pathway

被引:1060
作者
Smalle, J [1 ]
Vierstra, RD [1 ]
机构
[1] Univ Wisconsin, Dept Genet, Madison, WI 53706 USA
关键词
proteolysis; Arabidopsis; cell regulation; polypeptide tags;
D O I
10.1146/annurev.arplant.55.031903.141801
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Much of plant physiology, growth, and development is controlled by the selective removal of short-lived regulatory proteins. One important proteolytic pathway involves the small protein ubiquitin (Ub) and the 26S proteasome, a 2-MDa protease complex. In this pathway, Ub is attached to proteins destined for degradation; the resulting Ub-protein conjugates are then recognized and catabolized. by the 26S proteasome. This review describes our current understanding of the pathway in plants at the biochemical, genomic, and genetic levels, using Arabidopsis thaliana as the model. Collectively, these analyses show that the Ub/26S proteasome pathway is one of the most elaborate regulatory mechanisms in plants. The genome of Arabidopsis encodes more than 1400 (or >5% of the proteome) pathway components that can be connected to almost all aspects of its biology. Most pathway components participate in the Ub-ligation reactions that choose with exquisite specificity which proteins should be ubiquitinated. What remains to be determined is the identity of the targets, which may number in the thousands in plants.
引用
收藏
页码:555 / 590
页数:40
相关论文
共 189 条
[51]  
Genschik P, 1998, PLANT CELL, V10, P2063
[52]   HOMOLOGS OF THE ESSENTIAL UBIQUITIN CONJUGATING ENZYME-UBC1, ENZYME-4, AND ENZYME-5 IN YEAST ARE ENCODED BY A MULTIGENE FAMILY IN ARABIDOPOSIS-THALIANA [J].
GIROD, PA ;
CARPENTER, TB ;
VANNOCKER, S ;
SULLIVAN, ML ;
VIERSTRA, RD .
PLANT JOURNAL, 1993, 3 (04) :545-552
[53]  
Girod PA, 1999, PLANT CELL, V11, P1457
[54]   A subcomplex of the proteasome regulatory particle required for ubiquitin-conjugate degradation and related to the COP9-signalosome and eIF3 [J].
Glickman, MH ;
Rubin, DM ;
Coux, O ;
Wefes, I ;
Pfeifer, G ;
Cjeka, Z ;
Baumeister, W ;
Fried, VA ;
Finley, D .
CELL, 1998, 94 (05) :615-623
[55]   Auxin regulates SCFTIR1-dependent degradation of AUX/IAA proteins [J].
Gray, WM ;
Kepinski, S ;
Rouse, D ;
Leyser, O ;
Estelle, M .
NATURE, 2001, 414 (6861) :271-276
[56]   Arabidopsis SGT1b is required for SCFTIR1-mediated auxin response [J].
Gray, WM ;
Muskett, PR ;
Chuang, HW ;
Parker, JE .
PLANT CELL, 2003, 15 (06) :1310-1319
[57]   Role of the Arabidopsis RING-H2 protein RBX1 in RUB modification and SCF function [J].
Gray, WM ;
Hellmann, H ;
Dharmasiri, S ;
Estelle, M .
PLANT CELL, 2002, 14 (09) :2137-2144
[58]   The ubiquitin ligase activity in the DDB2 and CSA complexes is differentially regulated by the COP9 signalosome in response to DNA damage [J].
Groisman, R ;
Polanowska, J ;
Kuraoka, I ;
Sawada, J ;
Saijo, M ;
Drapkin, R ;
Kisselev, AF ;
Tanaka, K ;
Nakatani, Y .
CELL, 2003, 113 (03) :357-367
[59]   A gated channel into the proteasome core particle [J].
Groll, M ;
Bajorek, M ;
Köhler, A ;
Moroder, L ;
Rubin, DM ;
Huber, R ;
Glickman, MH ;
Finley, D .
NATURE STRUCTURAL BIOLOGY, 2000, 7 (11) :1062-1067
[60]   Structure of 20S proteasome from yeast at 2.4 angstrom resolution [J].
Groll, M ;
Ditzel, L ;
Lowe, J ;
Stock, D ;
Bochtler, M ;
Bartunik, HD ;
Huber, R .
NATURE, 1997, 386 (6624) :463-471