Stage-specific promoter activity from stably maintained episomes in Plasmodium falciparum

被引：13

作者：

Horrocks, P ^{[1
]}

Pinches, R ^{[1
]}

Kriek, N ^{[1
]}

Newbold, C ^{[1
]}

机构：

[1] Univ Oxford, John Radcliffe Hosp, Weatherall Inst Mol Med, Oxford OX3 9DS, England

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 2002年 / 32卷 / 10期

基金：

英国惠康基金;

关键词：

Plasmodium falciparum; transfection; transcriptional regulation;

D O I：

10.1016/S0020-7519(02)00123-6

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Genomic DNA is organised at its simplest level within phased arrays of nucleosomes, a structure key to the correct transcriptional regulation of the encoded genes. Here we studied chromatin formation on DNA transfected into Plasmodium falciparum either as an episomal plasmid or following integration by homologous recombination. We show that stably maintained and replicated plasmid assembles phased arrays of nucleosomes and that a reporter gene is transcribed in an appropriate temporal manner. These data provide a key observation for the future investigation of promoter structure and function with transfected DNA in Plasmodium spp. (C) 2002 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved.

引用

页码：1203 / 1206

页数：4

共 18 条

[1] PLASMODIUM-FALCIPARUM CHROMATIN - NUCLEOSOMAL ORGANIZATION AND HISTONE-LIKE PROTEINS [J].

CARY, C ;

LAMONT, D ;

DALTON, JP ;

DOERIG, C .

PARASITOLOGY RESEARCH, 1994, 80 (03) :255-258

[2] Malaria -: Cooperative silencing elements in var genes [J].

Deitsch, KW ;

Calderwood, MS ;

Wellems, TE .

NATURE, 2001, 412 (6850) :875-876

[3] Control of gene expression in Plasmodium falciparum [J].

Horrocks, P ;

Dechering, K ;

Lanzer, M .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1998, 95 (02) :171-181

[4]

HORROCKS P, 2002, IN PRESS MOL MICROBI

[5]

Horrocks Paul, 1999, Parasitology International, V48, P55, DOI 10.1016/S1383-5769(99)00002-1

[6] DNA replication-independent silencing in S-cerevisiae [J].

Kirchmaier, AL ;

Rine, J .

SCIENCE, 2001, 291 (5504) :646-650

[7] Precise timing of expression of a Plasmodium falciparum-derived transgene in Plasmodium berghei is a critical determinant of subsequent subcellular localization [J].

Kocken, CHM ;

van der Wel, AM ;

Dubbeld, MA ;

Narum, DL ;

van de Rijke, FM ;

van Gemert, GJ ;

van der Linde, X ;

Bannister, LH ;

Janse, C ;

Waters, AP ;

Thomas, AW .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (24) :15119-15124

[8] INTERPLAY BETWEEN CHROMATIN STRUCTURE AND TRANSCRIPTION [J].

KORNBERG, RD ;

LORCH, Y .

CURRENT OPINION IN CELL BIOLOGY, 1995, 7 (03) :371-375

[9] Chromatin-modifying and -remodeling complexes [J].

Kornberg, RD ;

Lorch, Y .

CURRENT OPINION IN GENETICS & DEVELOPMENT, 1999, 9 (02) :148-151

[10] A simple RNA analysis method shows var and rif multigene family expression patterns in Plasmodium falciparum [J].

Kyes, S ;

Pinches, R ;

Newbold, C .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2000, 105 (02) :311-315

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