Bis, a Bcl-2-binding protein that synergizes with Bcl-2 in preventing cell death

被引:180
作者
Lee, JH
Takahashi, T
Yasuhara, N
Inazawa, J
Kamada, S
Tsujimoto, Y [1 ]
机构
[1] Osaka Univ, Grad Sch Med, Biomed Res Ctr, Dept Med Genet, Osaka, Japan
[2] Japan Sci & Technol Corp, CREST, Suita, Osaka 5650871, Japan
[3] Tokyo Med & Dent Univ, Med Res Inst, Div Genet, Dept Mol Cytogenet,Bunkyo Ku, Tokyo 1138519, Japan
基金
日本学术振兴会;
关键词
apoptosis; Bcl-2; Bis;
D O I
10.1038/sj.onc.1203043
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bcl-2 is the best characterized inhibitor of apoptosis, although the molecular basis of this action is not fully understood. Using a protein interaction cloning procedure, we identified a human gene designated as bis (mapped to chromosome 10q25) that encoded a novel Bcl-2-interacting protein. Eis protein showed no significant homology with Bcl-2 family proteins and had no prominent functional motif. Co-immunoprecipitation analysis confirmed that Eis interacted with Bcl-2 in vivo. DNA transfection experiments indicated that Eis itself exerted only weak anti-apoptotic activity, but was synergistic with Bcl-2 in preventing flax-induced and Fas-mediated apoptosis. These results suggest that Bis is a novel modulator of cellular anti-apoptotic activity that functions through its interaction with Bcl-2.
引用
收藏
页码:6183 / 6190
页数:8
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