Cytokine profile in the liver of primary biliary cirrhosis

被引：108

作者：

Nagano, T ^{[1
]}

Yamamoto, K ^{[1
]}

Matsumoto, S ^{[1
]}

Okamoto, R ^{[1
]}

Tagashira, M ^{[1
]}

Ibuki, N ^{[1
]}

Matsumura, S ^{[1
]}

Yabushita, K ^{[1
]}

Okano, N ^{[1
]}

Tsuji, T ^{[1
]}

机构：

[1] Okayama Univ, Sch Med, Dept Internal Med 1, Okayama 700, Japan

来源：

JOURNAL OF CLINICAL IMMUNOLOGY | 1999年 / 19卷 / 06期

关键词：

primary biliary cirrhosis; cytokine; Th; 1; Th2;

D O I：

10.1023/A:1020511002025

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We characterized the cytokine profile in the liver of patients with primary biliary cirrhosis (PBC). Total RNA was extracted from the biopsy specimens of 9 patients with early-stage PBC, 10 with chronic hepatitis C (CHC), and 4 normal controls. cDNA was prepared and amplified with a polymerase chain reaction using primers for interferon (IFN)-gamma and interleukin (IL)-2, -4, -5, -6, -10, -12 (p40), and -15. Cytokines such as IFN-gamma and IL-5, -6, -10, -12, and -15 were expressed in most cases of PBC. Expression rates of IL-5 and IL-6 were higher than in CHC and controls. The higher expression rate of IL-5 in PBC was associated with eosinophil infiltration. IL-2 and IL-4 were rarely detected. Semiquantitative analysis revealed that the expression of IFN-gamma and IL-10 was reversed in PBC and CHC: high IFN-gamma and low IL-10 in PBC and high IL-10 and low IFN-gamma in CHC. These results suggest that cytokine expression is skewed in PBC and both Th1 and Th2 cytokines may play a role in the pathogenesis.

引用

页码：422 / 427

页数：6

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